Recombinant Mouse IL-36 alpha/IL-1F6 Protein

Name: Recombinant Mouse IL-36 alpha/IL-1F6 Protein
Brand: Novus Biologicals
SKU: NBP2-35128

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35128

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

Q9JLA2

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all IL-36 alpha/IL-1F6 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 160 amino acids corresponding to IL-36 alpha/IL-1F6 Source: E. coli

Uniprot ID: Q9JLA2

Amino Acid Sequence: MNKEKELRAA SPSLRHVQDL SSRVWILQNN ILTAVPRKEQ TVPVTITLLP CQYLDTLETN RGDPTYMGVQ RPMSCLFCTK DGEQPVLQLG EGNIMEMYNK KEPVKASLFY HKKSGTTSTF ESAAFPGWFI AVCSKGSCPL ILTQELGEIF ITDFEMIVVH

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of IL-36 alpha/IL-1F6 as determined by LAL method.

Predicted Molecular Mass

18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

IL36 alpha Protein is fully biologically active when compared to standard. The specific activity determined by its ability in a functional ELISA. Immobilized rMuIL-36alpha at 1 ug/mL can bind recombinant murine IL-1 Rrp2 with a range of 0.15-5 ug/mL.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse IL-36 alpha/IL-1F6 Protein

SDS-PAGE: Recombinant Mouse IL-36 alpha/IL-1F6 Protein [NBP2-35128]

SDS-Page: Mouse IL-36 alpha/IL-1F6 160 a.a Protein [NBP2-35128]

Formulation, Preparation and Storage

NBP2-35128

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	5% Trehalose
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IL-36 alpha/IL-1F6

Interleukin-36 (IL-36) is a pro-inflammatory cytokine which plays an important role in the pathophysiology of several diseases. IL-36alpha, IL-36beta, and IL-36gamma (formerly IL-1F6, IL-1F8, and IL-1F9) are IL-1 family members that signal through the IL-1 receptor family members IL-1Rrp2 (IL-1RL2) and IL-1RAcP. IL-36alpha is mainly found in skin and lymphoid tissues, but also in fetal brain, trachea, stomach and intestine. It is expressed by monocytes, B and T cells. Notably, IL-36 alpha is the only novel IL-1 family member expressed on T-cells. Recombinant murine interleukin-36 alpha contains 153 amino acids residues which is a single nonglycosylated polypeptide. Specifically, mouse IL-36alpha shares 83 % a.a. sequence identity with rat IL-36alpha, 54 - 60 % with human, rabbit, equine and bovine IL-36alpha.

Long Name

Interleukin 36 alpha/Interleukin 1 Family 6

Alternate Names

FIL1 epsilon, IL-1E, IL1F6, IL36 alpha, IL36A

Gene Symbol

IL36A

UniProt