Could you let us know the following information? 1) Lyophilized buffer formulation (e.g. Lyophilized from PBS.) 2) Preservative/Concentration

Name: Recombinant Human IL-36 beta/IL-1F8 Protein
Brand: Novus Biologicals
SKU: NBP2-34887

This protein is supplied in the lyophilized format from the same associated lab of ours, and can be used in the functional assays in vivo (so should not have any preservative and endotoxin-tested. Our lab's databases show that E. coli, lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, and no preservative.

Recombinant Human IL-36 beta/IL-1F8 Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34887

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Product Datasheet / COA

Catalog #

Availability

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all IL-36 beta/IL-1F8 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 157 amino acids corresponding to IL-36 beta/IL-1F8 Source: E. coli

Uniprot ID: Q9NZH7-2

Amino Acid Sequence: MNPQREAAPK SYAIRDSRQM VWVLSGNSLI AAPLSRSIKP VTLHLIACRD TEFSDKEKGN MVYLGIKGKD LCLFCAEIQG KPTLQLKEKN IMDLYVEKKA QKPFLFFHNK EGSTSVFQSV SYPGWFIATS TTSGQPIFLT KERGITNNTN FYLDSVE

This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of IL-36 beta/IL-1F8 as determined by LAL method.

Predicted Molecular Mass

17.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

IL-36 beta/IL-1F8 protein is fully biologically active when compared to standard. The specific activity is determined by its binding ability in a functional ELISA. Immobilized IL-36 beta/IL-1F8 at 1 ug/mL can bind recombinant human IL-1 Rrp2 Fc Chimera with a range of 0.15-5 ug/mL.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human IL-36 beta/IL-1F8 Protein

SDS-PAGE: Recombinant Human IL-36 beta/IL-1F8 Protein [NBP2-34887]

SDS-Page: Human IL-36 beta/IL-1F8 157 a.a Protein [NBP2-34887]

Formulation, Preparation and Storage

NBP2-34887

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IL-36 beta/IL-1F8

Interleukin-1 beta (IL-1B) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-1a and IL-1B binds to the same receptor and has similar but not identical biological properties; The mature human IL-1B shares 96% amino acid sequence identity with rhesus and 67 % -78 % with canine, mouse and rat IL-1B.

Long Name

Interleukin 36 beta/Interleukin 1 Family 8

Alternate Names

FIL1 eta, IL-1 eta, IL-1H2, IL1F8, IL36 beta, IL36B

Gene Symbol

IL36B

Additional IL-36 beta/IL-1F8 Products

Product Documents for Recombinant Human IL-36 beta/IL-1F8 Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Human IL-36 beta/IL-1F8 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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