Recombinant Mouse IL-36 beta/IL-1F8 Protein

Name: Recombinant Mouse IL-36 beta/IL-1F8 Protein
Brand: Novus Biologicals
SKU: NBP2-35129

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35129

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Product Specifications
Scientific Data
Preparation & Storage
Background
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Specific Notices

Key Product Details

Source

E. coli

Accession #

Q9D6Z6

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all IL-36 beta/IL-1F8 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 153 amino acids corresponding to IL-36 beta/IL-1F8 Source: E. coli

Uniprot ID: Q9D6Z6

Amino Acid Sequence: SSQSPRNYRV HDSQQMVWVL TGNTLTAVPA SNNVKPVILS LIACRDTEFQ DVKKGNLVFL GIKNRNLCFC CVEMEGKPTL QLKEVDIMNL YKERKAQKAF LFYHGIEGST SVFQSVLYPG WFIATSSIER QTIILTHQRG KLVNTNFYIE SEK

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of IL-36 beta/IL-1F8 as determined by LAL method.

Predicted Molecular Mass

17.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

IL36 Beta Protein is fully biologically active when compared to standard. The ED50 as determined by inducing IL-6 secretion in murine NIH/3T3 cells is less than 10 ng/ml, corresponding to a specific activity of > 1.0 x 10^5 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse IL-36 beta/IL-1F8 Protein

SDS-PAGE: Recombinant Mouse IL-36 beta/IL-1F8 Protein [NBP2-35129]

SDS-Page: Mouse IL-36 beta/IL-1F8 153 a.a Protein [NBP2-35129]

Formulation, Preparation and Storage

NBP2-35129

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	5% Trehalose
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IL-36 beta/IL-1F8

Interleukin-36 is a pro-inflammatory cytokine which plays an important role in the pathophysiology of several diseases. IL-36alpha, IL-36beta, and IL-36gamma (formerly IL-1F6, IL-1F8, and IL-1F9) are IL-1 family members that signal through the IL-1 receptor family members IL-1Rrp2 (IL-1RL2) and IL-1RAcP. IL-36 beta is reported to be expressed at higher levels in psoriatic plaques than in symptomless psoriatic skin or healthy control skin and it can stimulate production of interleukin-6 and interleukin-8 in synovial fibrobasts, articular chondrocytes and mature adipocytes. IL-36 beta has two isoforms. IL-36beta2 contains one potential N-linked glycosylation site in its C-terminus, while IL -36beta isoform 1 lacks potential N-linked glycosylation sites and four of the conserved beta-strands. Within the IL-1 family, IL-36beta/IL-1F8 shares 30 %, 32 %, 37 %, 46 %, 34 %, 45 % and 28 % aa sequence identity with IL-1 ra, IL-1beta, IL-36Ra/IL-1F5, IL-36alpha/IL-1F6, IL-37/IL-1F7, IL-36gamma/IL-1F9 and IL-1F10, respectively.

Long Name

Interleukin 36 beta/Interleukin 1 Family 8

Alternate Names

FIL1 eta, IL-1 eta, IL-1H2, IL1F8, IL36 beta, IL36B

Gene Symbol

IL36B

UniProt