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Recombinant Mouse IL-36 gamma/IL-1F9 Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-41388

Novus Biologicals, part of Bio-Techne
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NBP2-41388-0.01mg
NBP2-41388-1000ug

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 152 amino acids corresponding to IL-36 gamma/IL-1F9 Source: E. coli

Uniprot ID: Q8R460

Amino Acid Sequence: GRETPDFGEV FDLDQQVWIF RNQALVTVPR SHRVTPVSVT ILPCKYPESL EQDKGIAIYL GIQNPDKCLF CKEVNGHPTL LLKEEKILDL YHHPEPMKPF LFYHTRTGGT STFESVAFPG HYIASSKTGN PIFLTSKKGE YYNINFNLDI KS

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of IL-36 gamma/IL-1F9 as determined by LAL method.

Predicted Molecular Mass

17.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Fully biologically active when compared to standard. The ED50 as determined by inducing IL-6 secretion in murine NIH/3T3 cells is less than 10 ng/ml, corresponding to a specific activity of > 1.0 x 10^5 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse IL-36 gamma/IL-1F9 Protein

SDS-PAGE: Recombinant Mouse IL-36 gamma/IL-1F9 Protein [NBP2-41388]

SDS-PAGE: Recombinant Mouse IL-36 gamma/IL-1F9 Protein [NBP2-41388]

SDS-Page: Mouse IL-36 gamma/IL-1F9 152 a.a Protein [NBP2-41388]

Formulation, Preparation and Storage

NBP2-41388
Formulation Lyophilized from a 0.2 um filtered solution in 1 M MOPS, 10 mM NaAC, pH7.6, 2 mM EDTA, 0.02 % Tween-20.
Preservative 5% Trehalose
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IL-36 gamma/IL-1F9

The protein encoded by this gene is a member of the interleukin 1 cytokine family. The activity of this cytokine is mediated by interleukin 1 receptor-like 2 (IL1RL2/IL1R-rp2), and is specifically inhibited by interleukin 1 family, member 5 (IL1F5/IL-1 delta). Interferon-gamma, tumor necrosis factor-alpha and interleukin 1, beta (IL1B) are reported to stimulate the expression of this cytokine in keratinocytes. The expression of this cytokine in keratinocytes can also be induced by a contact hypersensitivity reaction or herpes simplex virus infection. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.

Long Name

Interleukin 36 gamma/Interleukin 1 Family 9

Alternate Names

IL-1 H1, IL-1rp2, IL1F9, IL36 gamma, IL36G

Gene Symbol

IL36G

UniProt

Additional IL-36 gamma/IL-1F9 Products

Product Documents for Recombinant Mouse IL-36 gamma/IL-1F9 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Mouse IL-36 gamma/IL-1F9 Protein

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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