Recombinant Rat IL-5 Protein

Name: Recombinant Rat IL-5 Protein
Brand: Novus Biologicals
SKU: NBP2-35253

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35253

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

Q08125

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all IL-5 Proteins and Enzymes »

Product Specifications

Description

A disulfide-linked homodimeric protein containing two 113 amino acid chains corresponding to IL-5 Source: E. coli

Uniprot ID: Q08125

Amino Acid Sequence: MEIPMSTVVK ETLIQLSTHR ALLTSNETMR LPVPTHKNHQ LCIGEIFQGL DILKNQTVRG GTVEILFQNL SLIKKYIDGQ KEKCGEERRK TRHFLDYLQE FLGVMSTEWA MEV

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

> 98 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of IL-5 as determined by LAL method.

Predicted Molecular Mass

26.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

IL-5 protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 x 10^6 IU/mg

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Rat IL-5 Protein

SDS-PAGE: Recombinant Rat IL-5 Protein [NBP2-35253]

SDS-Page: Rat IL-5 Protein [NBP2-35253]

HPLC: Recombinant Rat IL-5 Protein [NBP2-35253]

HPLC: Recombinant Rat IL-5 Protein [NBP2-35253] - Rat IL5 Protein [NBP2-35253]

Formulation, Preparation and Storage

NBP2-35253

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IL-5

IL-5, also named B-cell differentiation factor I, eosinophil differentiation factor and TRF, is belonging to the cytokine family and the IL-5 gene is in close proximity to the genes encoding IL-3, IL-4, and granulocyte-macrophage colony-stimulating factor (GM-CSF), which are often co-expressed in TH2 cells. Through binding to the IL-5 receptor, IL-5 stimulates B cell growth and increases immunroglobulin secretion. It is also a key mediator in eosinophil activation. Interleukin-5 has long been associated with the cause of several allergic diseases including allergic rhinitis and asthma.Rat IL-5 is a 132-amino acid (115 in human, 133 in the mouse) -long TH2 cytokine that is part of the hematopoietic family. Unlike other members of this cytokine family (namely IL-3 and GM-CSF), this glycoprotein in its active form is a homodimer.

Long Name

Interleukin 5

Alternate Names

BCDF mu, BCGFII, EDF, Eo-CSF, IL5, TRF

Gene Symbol

IL5

UniProt