Recombinant Human IL-7 Protein

Name: Recombinant Human IL-7 Protein
Brand: Novus Biologicals
SKU: NBP2-34902

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34902

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Product Specifications
Scientific Data
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Background
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Key Product Details

Source

E. coli

Accession #

P13232

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all IL-7 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 152 amino acids corresponding to IL-7 Source: E. coli

Uniprot ID: P13232

Amino Acid Sequence: DCDIEGKDGK QYESVLMVSI DQLLDSMKEI GSNCLNNEFN FFKRHICDAN KEGMFLFRAA RKLRQFLKMN STGDFDLHLL KVSEGTTILL NCTGQVKGRK PAALGEAQPT KSLEENKSLK EQKKLNDLCF LKRLLQEIKT CWNKILMGTK EH

This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of IL-7 as determined by LAL method.

Predicted Molecular Mass

17.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Fully biologically active when compared to standard. IL-7 stimulates proliferation of PHA-activated human peripheral blood mononuclear cell (PBMC). The specific activity of Recombinant Human IL-7 is > 1.0 x 10^8 Units/mg

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human IL-7 Protein

SDS-PAGE: Recombinant Human IL-7 Protein [NBP2-34902]

SDS-Page: Human IL-7 Protein [NBP2-34902] - MW= Approximately 17 kDa

Formulation, Preparation and Storage

NBP2-34902

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PBS, 150 mM NaCl, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IL-7

Interleukin-7 (IL-7) is encoded by the IL7 gene and secreted by stromal cells in the red marrow and thymus. It binds to the IL-7 receptor, a heterodimer consisting of IL-7 receptor alpha and IL-2 receptor gamma chain. IL-7 stimulates the differentiation of hematopoietic stem cells into lymphoid progenitor cells and also stimulates proliferation of B cells, T cells and NK cells. Murine IL-7 has approximately 65 % amino acid sequence identity with human IL-7 and both proteins exhibit cross-species activity. IL-7 as an immunotherapy agent has been examined in many human clinical trials for various malignancies and during HIV infection

Long Name

Interleukin 7

Alternate Names

IL7, Lymphopoietin-1, PBGF

Gene Symbol

IL7

UniProt