Recombinant Rat IL-7 Protein

Name: Recombinant Rat IL-7 Protein
Brand: Novus Biologicals
SKU: NBP2-35255

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35255

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P56478

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all IL-7 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 129 amino acids corresponding to IL-7 Source: E. coli

Uniprot ID: P56478

Amino Acid Sequence: DCHIKDKDGK AFGSVLMISI NQLDKMTGTD SDCPNNEPNF FKKHLCDDTK EAAFLNRAAR KLRQFLKMNI SEEFNDHLLR VSDGTQTLVN CTSKEEKTIK EQKKNDPCFL KRLLREIKTC WNKILKGSI

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of IL-7 as determined by LAL method.

Predicted Molecular Mass

14.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

IL7 Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine 2E8 cells is less than 2.0 ng/ml, corresponding to a specific activity of > 5.0 x 10^5 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Rat IL-7 Protein

SDS-PAGE: Recombinant Rat IL-7 Protein [NBP2-35255]

SDS-Page: Rat IL-7 Protein [NBP2-35255]

Formulation, Preparation and Storage

NBP2-35255

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IL-7

Interleukin-7 (IL-7) is encoded by the IL7 gene and secreted by stromal cells in the red marrow and thymus. It binds to the IL-7 receptor, a heterodimer consisting of IL-7 receptor alpha and IL-2 receptor gamma chain. IL-7 stimulates the differentiation of hematopoietic stem cells into lymphoid progenitor cells and also stimulates proliferation of B cells, T cells and NK cells. Furthermore, IL-7 as an immunotherapy agent has been examined in many human clinical trials for various malignancies and during HIV infection. Rat IL-7 contains 129 amino acid residues and has three disulfide bonds. In addition, it has approximately 57 % and 88 % amino acid sequence identity with human and murine IL-7.

Long Name

Interleukin 7

Alternate Names

IL7, Lymphopoietin-1, PBGF

Gene Symbol

IL7

UniProt

P56478 (Rat)

Additional IL-7 Products

Product Documents for Recombinant Rat IL-7 Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Rat IL-7 Protein

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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