Recombinant Rat IL-9 Protein

Name: Recombinant Rat IL-9 Protein
Brand: Novus Biologicals
SKU: NBP2-35257

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35257

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Product Specifications
Scientific Data
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Background
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Specific Notices

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all IL-9 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 127 amino acids corresponding to IL-9 Source: E. coli

Uniprot ID: D4A8I9

Amino Acid Sequence: MQRCSTSWGI QHTSYLIENL KDDPSSKCSC SANVTSCLCL PIPSDDCTTP CFQEGMSQVT NATQQSKFSP FFFRVKRIVE TLKSNKCQFF SCEKPCNQTT AGNTVSFLKS LLKTFQKTEV QVQRSRA

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of IL-9 as determined by LAL method.

Predicted Molecular Mass

14.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

IL9 Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine TS1 cells is less than 10 ng/ml, corresponding to a specific activity of > 1.0 x 10^5 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Rat IL-9 Protein

SDS-PAGE: Recombinant Rat IL-9 Protein [NBP2-35257]

SDS-Page: Rat IL-9 Protein [NBP2-35257]

Formulation, Preparation and Storage

NBP2-35257

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IL-9

Interleukin-9 (IL-9) is encoded by the IL9 gene and produced by T-cells and specifically by CD4+ helper cells. IL-9 was originally identified as a cytokine found in the conditioned medium of a human T cell leukemia virus type I (HTLVI) transformed T cell line. It functions through the IL-9 receptor, which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes. IL-9 can support the growth of IL-2 independent and IL- 4 independent helper T-cells. Rat IL-9 has approximately 73 % amino acid sequence identity with murine IL-9. The gene encoding this cytokine has been identified as a candidate gene for asthma. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a determining factor in the pathogenesis of bronchial hyperresponsiveness.

Long Name

Interleukin 9

Alternate Names

IL9

Gene Symbol

IL9

Additional IL-9 Products

Product Documents for Recombinant Rat IL-9 Protein

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COA

Product Specific Notices for Recombinant Rat IL-9 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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