Recombinant Human IVD His Protein

Name: Recombinant Human IVD His Protein
Brand: Novus Biologicals
SKU: NBP1-40406

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-40406

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

NP_002216.2

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all IVD Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 33-426 of Human IVD

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MHSLLPVDDA INGLSEEQRQ LRQTMAKFLQ EHLAPKAQEI DRSNEFKNLR EFWKQLGNLG VLGITAPVQY GGSGLGYLEH VLVMEEISRA SGAVGLSYGA HSNLCINQLV RNGNEAQKEK YLPKLISGEY IGALAMSEPN AGSDVVSMKL KAEKKGNHYI LNGNKFWITN GPDADVLIVY AKTDLAAVPA SRGITAFIVE KGMPGFSTSK KLDKLGMRGS NTCELIFEDC KIPAANILGH ENKGVYVLMS GLDLERLVLA GGPLGLMQAV LDHTIPYLHV REAFGQKIGH FQLMQGKMAD MYTRLMACRQ YVYNVAKACD EGHCTAKDCA GVILYSAECA TQVALDGIQC FGGNGYINDF PMGRFLRDAK LYEIGAGTSE VRRLVIGRAF NADFH

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

45.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human IVD His Protein

SDS-PAGE: Recombinant Human IVD His Protein [NBP1-40406]

SDS-Page: IVD Protein [NBP1-40406] - IVD, 45.3 kDa (415aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE

Formulation, Preparation and Storage

NBP1-40406

Formulation	20 mM Tris-HCl buffer (pH8.0), 1 mM DTT, 10% glycerol
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IVD

IVD (Isovaleryl Coenzyme A dehydrogenase) is a mitochondrial matrix enzyme that belongs to the acyl-CoA dehydrogenase family. IVD is a homotetrameric flavoenzyme which catalyzes the conversion of isovaleryl-CoA to 3-methylcrotonyl-CoA. Defects of the IVD gene lead to ineffective isoforms that are the underlying cause of isovaleric acidemia. Recombinant human IVD protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

ACAD2, EC 1.3.99, EC 1.3.99.10, FLJ12715, FLJ34849, isovaleryl-CoA dehydrogenase, mitochondrial

Gene Symbol

IVD

UniProt

NP_002216.2 (Human)

Additional IVD Products

Product Documents for Recombinant Human IVD His Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Human IVD His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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