KLF15 Antibody Blocking Peptide

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24635PEP

Novus Biologicals, part of Bio-Techne
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Key Product Details

Conjugate

Unconjugated

Applications

Antibody Competition

View all KLF15 Proteins and Enzymes »

Product Specifications

Description

Application Notes

This peptide is useful as a blocking peptide for NBP2-24635. For further blocking peptide related protocol, click here.

Specificity

This peptide is specific for NBP2-24635 only.

Protein / Peptide Type

Antibody Blocking Peptide

Formulation, Preparation and Storage

NBP2-24635PEP
Formulation Peptide dissolved in dH20. Contains no BSA.
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -80C. Avoid freeze-thaw cycles.

Background: KLF15

The kidney chloride channel proteins CLCNKA and CLCNKB are highly related, although they are located in different areas of the kidney. Using a yeast 1-hybrid screen of a kidney cDNA library with the GA element of the CLCNKA promoter as bait, Uchida et al. (2000) isolated cDNAs encoding MAZ and KLF15, which they termed KKLF. Sequence analysis predicted that the 415-amino acid KLF15 protein, which is 84% identical to the rat Klf15 protein, contains 3 zinc finger motifs at its C terminus, N-terminal serine-rich stretches, and a central proline-rich segment. EMSA analysis confirmed that KLF15 and MAZ interact with the GA element of the CLCNKA promoter and showed that KLF15 binds with higher affinity and is a functional competitor of MAZ. Northern blot analysis revealed highest expression of a 2.5-kb KLF15 transcript in liver, followed by heart, skeletal muscle, and kidney. No expression was found in bone marrow or lymphoid tissues. Western blot analysis showed expression of a 50-kD protein. Immunohistochemical analysis detected nuclear expression of KLF15 in liver sinusoid stellate cells, subcapsular fibroblasts, and portal fibroblasts. KLF15 was also expressed in cardiac and skeletal muscle interstitial cells and in kidney inner medulla, glomeruli, and cortical interstitium. Immunofluorescence microscopy, however, demonstrated no colocalization of KLF15 with CLCNKA.

Alternate Names

DKFZp779M1320, kidney-enriched Kruppel-like factor, KKLFKidney-enriched krueppel-like factor, Krueppel-like factor 15, Kruppel-like factor 15

Gene Symbol

KLF15

Additional KLF15 Products

Product Documents for KLF15 Antibody Blocking Peptide

Product Datasheets

Certificate of Analysis

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Product Specific Notices for KLF15 Antibody Blocking Peptide

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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