Recombinant Human Leptin/OB Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34934

Novus Biologicals, part of Bio-Techne
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NBP2-34934-1mg
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Key Product Details

Source

E. coli

Accession #

P41159

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all Leptin/OB Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 146 amino acids corresponding to Leptin/OB Source: E. coli

Uniprot ID: P41159

Amino Acid Sequence: VPIQKVQDDT KTLIKTIVTR INDISHTQSV SSKQKVTGLD FIPGLHPILT LSKMDQTLAV YQQILTSMPS RNVIQISNDL ENLRDLLHVL AFSKSCHLPW ASGLETLDSL GGVLEASGYS TEVVALSRLQ GSLQDMLWQL DLSPGC

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of Leptin/OB as determined by LAL method.

Predicted Molecular Mass

16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Leptin Protein is fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human Leptin R transfected BaF3 murine proB cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 x 10^5 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Leptin/OB Protein

SDS-PAGE: Recombinant Human Leptin/OB Protein [NBP2-34934]

SDS-PAGE: Recombinant Human Leptin/OB Protein [NBP2-34934]

SDS-Page: Human Leptin/OB Protein [NBP2-34934]

Formulation, Preparation and Storage

NBP2-34934
Formulation Lyophilized from a 0.2 um filtered concentrated solution in 50 mm PB, pH 3.5, with 0.02 % Tween-20.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: Leptin/OB

Human Leptin plays a key role in regulating energy intake and energy expenditure, including appetite and metabolism. It is one of the most important adipose derived hormones. The Ob (Lep) gene (Ob for obese, Lep for leptin) is located on chromosome 7 in humans. The protein is manufactured primarily in the adipocytes of white adipose tissue, and the level of circulating leptin is directly proportional to the total amount of fat in the body. Leptin acts on receptors in the hypothalamus of the brain where it inhibits appetite by (1) counteracting the effects of neuropeptide Y (a potent feeding stimulant secreted by cells in the gut and in the hypothalamus); (2) counteracting the effects of anandamide (another potent feeding stimulant that binds to the same receptors as THC), and (3) promoting the synthesis of alpha-MSH, an appetite suppressant. This appetite inhibition is long-term, in contrast to the rapid inhibition of eating by cholecystokinin (CCK) and the slower suppression of hunger between meals mediated by PYY3- 36. The absence of leptin (or its receptor) leads to uncontrolled food intake and resulting obesity.

Alternate Names

LEP, OB

Gene Symbol

LEP

UniProt

P41159

Additional Leptin/OB Products

Product Documents for Recombinant Human Leptin/OB Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human Leptin/OB Protein

This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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