Recombinant Human LIF Protein

Name: Recombinant Human LIF Protein
Brand: Novus Biologicals
SKU: NBP2-34935

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34935

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P15018

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all LIF Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 180 amino acids corresponding to LIF Source: E. coli

Uniprot ID: P15018

Amino Acid Sequence: SPLPITPVNA TCAIRHPCHN NLMNQIRSQL AQLNGSANAL FILYYTAQGE PFPNNLDKLC GPNVTDFPPF HANGTEKAKL VELYRIVVYL GTSLGNITRD QKILNPSALS LHSKLNATAD ILRGLLSNVL CRLCSKYHVG HVDVTYGPDT SGKDVFQKKK LGCQLLGKYK QIIAVLAQAF

Purity

> 98 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of LIF as determined by LAL method.

Predicted Molecular Mass

19.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

LIF Protein is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent proliferation of human TF-1 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 x 10^7 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human LIF Protein

SDS-PAGE: Recombinant Human LIF Protein [NBP2-34935]

SDS-Page: Human LIF Protein [NBP2-34935]

HPLC: Recombinant Human LIF Protein [NBP2-34935]

HPLC: Recombinant Human LIF Protein [NBP2-34935] - man LIF Protein [NBP2-34935]

Formulation, Preparation and Storage

NBP2-34935

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-0.2mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: LIF

Leukemia inhibitory factor (LIF) is a member of Interleukin 6 family. This protein is mainly expressed in the trophectoderm of the developing embryo, with its receptor LIFR expressed throughout the inner cell mass. LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. LIF is used in mouse embryonic stem cell culture, because that removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. It is also used in phase II clinical trial, which can assist embryo implantation in women who have failed to become pregnant despite assisted reproductive technologies (ART). Mature human LIF (180 a.a.) shares 78 %, 82 %, 91 %, 88 % and 87 % a.a. sequence identity with mouse, rat, canine, bovine, and porcine LIF, respectively.

Long Name

Leukemia Inhibitory Factor

Alternate Names

D Factor, Emfilermin, HILDA, MLPLI

Gene Symbol

LIF

UniProt