Recombinant Rabbit LIF Protein

Name: Recombinant Rabbit LIF Protein
Brand: Novus Biologicals
SKU: NBP3-11064

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11064

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Accession #

U3KLT1

Conjugate

Unconjugated

Applications

ELISA, Western Blot

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Product Specifications

Description

Recombinant Rabbit Leukemia Inhibitory Factor (LIF) is a recombinant protein corresponding to aa 23-202 of native rabbit LIF.

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

19.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Rabbit LIF Protein

ELISA: Recombinant Rabbit LIF Protein [NBP3-11064]

ELISA: Recombinant Rabbit LIF Protein [NBP3-11064] - Recombinant Rabbit Leukemia Inhibitory Factor detected using Chicken anti Rabbit Leukemia Inhibitory Factor as the capture reagent and Chicken anti Rabbit Leukemia Inhibitory Factor:Biotin as the detection reagent followed by Streptavidin:HRP.

Formulation, Preparation and Storage

NBP3-11064

Preparation Method	Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography.
Formulation	PBS
Preservative	10% Trehalose
Concentration	Lyoph
Reconstitution	Reconstitute with 20 uL sterile PBS containing 0.1% carrier protein. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. We recommend that the vial is gently mixed after reconstitution.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20C. Avoid freeze-thaw cycles.

Background: LIF

LIF (leukemia inhibitory factor, myeloid leukemia inhibitory factor) a pleiotropic lymphoid factor was initially described as a factor that inhibits the proliferation of myeloid leukemia cells and induces their differentiation into macrophages. Other activities of LIF include inhibition of adipogenesis, cholinergic neuron differentiation and bone metabolism. Human and mouse LIF share 78% homology and activities of LIF are not species-specific. Human LIF is active on mouse cells, but murine LIF is not active on human cells. Benefits of NovActive® Proteins

Fully Active: -Fully folded naturally by eukaryotic source; -Comparable for eukaryotic glycosylation
BioRisk Free: -animal-free; -serum-free; -endotoxin-free; -infection reagent-free; -low in proteolyic activity; -low in pyrogenic and pro-inflammatory activity
Just reconstitute and add to your cell culture.
Have FDA G.R.A.S. (generally recognized as safe) approval.

Advantages of barley endosperm Folding -Proficient protein machinery, with eukaryotic folding, allows for native formation of the protein and long term protection and storage. Environment -A biochemically inert environment, void of endotoxins, low protease activity and secondary metabolite content, and a simple protein profile, aid in downstream processing in barley. FDA G.R.A.S status -Barley is recognized by the FDA as having G.R.A.S (generally recognized as safe) status.

Long Name

Leukemia Inhibitory Factor

Alternate Names

D Factor, Emfilermin, HILDA, MLPLI

Gene Symbol

LIF

UniProt

U3KLT1 (Rabbit)

Additional LIF Products

Product Documents for Recombinant Rabbit LIF Protein

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COA

Product Specific Notices for Recombinant Rabbit LIF Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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