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Recombinant Human LMW-PTP/ACP1 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBC1-21279

Novus Biologicals, part of Bio-Techne
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NBC1-21279

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

Enzyme Activity, SDS-PAGE

Product Specifications

Description

A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-158 of Human LMW-PTP/ACP1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAEQATKSVL FVCLGNICRS PIAEAVFRKL VTDQNISENW VIDSGAVSDW NVGRSPDPRA VSCLRNHGIH TAHKARQITK EDFATFDYIL CMDESNLRDL NRKSNQVKTC KAKIELLGSY DPQKQLIIED PYYGNDSDFE TVYQQCVRCC RAFLEKAH

Purity

>95%, by SDS-PAGE

Endotoxin Level

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Predicted Molecular Mass

20.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is > 15,000 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human LMW-PTP/ACP1 His Protein

SDS-PAGE: Recombinant Human LMW-PTP/ACP1 His Protein [NBC1-21279]

SDS-PAGE: Recombinant Human LMW-PTP/ACP1 His Protein [NBC1-21279]

SDS-Page: Recombinant Human LMW-PTP/ACP1 Protein [NBC1-21279] - Human, His-tagged, Recombinant, E.coli

Formulation, Preparation and Storage

NBC1-21279
Formulation 20 mM MES (pH 6.0), 0.1 mM PMSF, 2 mM EDTA, 10% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LMW-PTP/ACP1

Acid phosphatase 1, soluble, also known as ACP1, belongs to the phosphotyrosine protein. It functions as an acid phosphatase and a protein tyrosine phosphatase (PTPase) present in all human tissues, including adipocytes. This enzyme hydrolyzes protein tyrosine phosphate to protein tyrosine and orthophosphate, and also orthophosphoric monoesters to alcohol and orthophosphate. Recombinant ACP1, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography techniques.

Long Name

Low Molecular Weight Phosphotyrosine Protein Phosphotase

Alternate Names

ACP1, HAAP, LMWPTP

Gene Symbol

ACP1

Additional LMW-PTP/ACP1 Products

Product Documents for Recombinant Human LMW-PTP/ACP1 His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human LMW-PTP/ACP1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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