I was wondering if your Lysyl tRNA synthetase Protein (NBP1-72410) is active or not? I'm looking to use active human tRNA synthetase for an experiment. Thank you.

Name: Recombinant Human Lysyl tRNA synthetase His Protein
Brand: Novus Biologicals
SKU: NBP1-72410

Unfortunately, this protein lysate has never been tested in any functional assay. Furthermore, this protein was linked to a His-tag and produced in E. coli, where there has no proper post-translation modification that could render the functional protein product. Therefore, we do not beleive that this protein may have functional activity.

Recombinant Human Lysyl tRNA synthetase His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-72410

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Lysyl tRNA synthetase Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 63-597 of Human Lysyl tRNA synthetase

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSGVGPEEE SVDPNQYYKI RSQAIHQLKV NGEDPYPHKF HVDISLTDFI QKYSHLQPGD HLTDITLKVA GRIHAKRASG GKLIFYDLRG EGVKLQVMAN SRNYKSEEEF IHINNKLRRG DIIGVQGNPG KTKKGELSII PYEITLLSPC LHMLPHLHFG LKDKETRYRQ RYLDLILNDF VRQKFIIRSK IITYIRSFLD ELGFLEIETP MMNIIPGGAV AKPFITYHNE LDMNLYMRIA PELYHKMLVV GGIDRVYEIG RQFRNEGIDL THNPEFTTCE FYMAYADYHD LMEITEKMVS GMVKHITGSY KVTYHPDGPE GQAYDVDFTP PFRRINMVEE LEKALGMKLP ETNLFETEET RKILDDICVA KAVECPPPRT TARLLDKLVG EFLEVTCINP TFICDHPQIM SPLAKWHRSK EGLTERFELF VMKKEICNAY TELNDPMRQR QLFEEQAKAK AAGDDEAMFI DENFCTALEY GLPPTAGWGM GIDRVAMFLT DSNNIKEVLL FPAMKPEDKK ENVATTDTLE STTVGTSV

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

63.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Lysyl tRNA synthetase His Protein

SDS-PAGE: Recombinant Human Lysyl tRNA synthetase His Protein [NBP1-72410]

SDS-Page: Recombinant Human Lysyl tRNA synthetase Protein [NBP1-72410] - 15% SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-72410

Formulation	20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1 M NaCl, 1 mM DTT
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lysyl tRNA synthetase

KARS, also known as Lysyl-tRNA synthetase, belongs to the class-II aminoacyl-tRNA synthetase family. It exists as both mitochondrial and cytoplasmic isoforms produced by alternative splicing, and thought to play a role in autoimmune diseases, such as polymyositis or dermatomyositis. This protein functions to catalyze the aminoacylation of tRNAs by their corresponding amino acids, thus linking amino acids with tRNA-contained nucleotide triplets. Recombinant human KARS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Alternate Names

CMTRIB, EC 6.1.1, EC 6.1.1.6, KARS2, KIAA0070, KRS, lysine tRNA ligase, Lysine--tRNA ligase, LysRS, lysyl-tRNA synthetase

Gene Symbol

KARS1

Additional Lysyl tRNA synthetase Products

Product Documents for Recombinant Human Lysyl tRNA synthetase His Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Human Lysyl tRNA synthetase His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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