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Recombinant Human MAD3 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23219

Novus Biologicals, part of Bio-Techne
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NBP2-23219

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-206 of Human MAD3

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMEPLASN IQVLLQAAEF LERREREAEH GYASLCPHRS PGPIHRRKKR PPQAPGAQDS GRSVHNELEK RRRAQLKRCL ERLKQQMPLG ADCARYTTLS LLRRARMHIQ KLEDQEQRAR QLKERLRSKQ QSLQRQLEQL RGLAGAAERE RLRADSLDSS GLSSERSDSD QEELEVDVES LVFGGEAELL RGFVAGQEHS YSHGGGAWL

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

25.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human MAD3 His Protein

SDS-PAGE: Recombinant Human MAD3 His Protein [NBP2-23219]

SDS-PAGE: Recombinant Human MAD3 His Protein [NBP2-23219]

SDS-Page: MAD3 Protein [NBP2-23219]

Formulation, Preparation and Storage

NBP2-23219
Formulation 20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MAD3

MXD3 is a member of the Myc superfamily of basic helix-loop-helix leucine zipper transcriptional regulators. The protein forms a heterodimer with the cofactor MAX which binds specific E-box DNA motifs in the promoters of target genes and regulates their transcription. Disruption of the MAX-MXD3 complex is associated with uncontrolled cell proliferation and tumorigenesis. Transcript variants of this gene encoding different isoforms have been described. Recombinant human MXD3 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Alternate Names

BHLHC13, Class C basic helix-loop-helix protein 13, MAD3FLJ35523, MAX dimerization protein 3, Max dimerizer 3, Max-associated protein 3, Max-interacting transcriptional repressor MAD3, MGC2383, MYX

Gene Symbol

MXD3

Additional MAD3 Products

Product Documents for Recombinant Human MAD3 His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human MAD3 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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