Recombinant Human MCEE His Protein

Name: Recombinant Human MCEE His Protein
Brand: Novus Biologicals
SKU: NBP1-49440

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49440

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Technical Data
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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all MCEE Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 37-176 of Human MCEE

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MQVTGSVWNL GRLNHVAIAV PDLEKAAAFY KNILGAQVSE AVPLPEHGVS VVFVNLGNTK MELLHPLGRD SPIAGFLQKN KAGGMHHICI EVDNINAAVM DLKKKKIRSL SEEVKIGAHG KPVIFLHPKD CGGVLVELEQ A

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

17.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human MCEE His Protein

SDS-PAGE: Recombinant Human MCEE His Protein [NBP1-49440]

SDS-Page: Recombinant Human MCEE Protein [NBP1-49440] - 15 % SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-49440

Formulation	20 mM Tris-HCl buffer (pH8.0), 0.2 M NaCl, 1 mM DTT, 0.1 mM PMSF, 10% glycerol
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MCEE

MCEE catalyzes the interconversion of D- and L-methylmalonyl-CoA during the degradation of branched chain amino acids, odd chain-length fatty acids, and other metabolites. This protein deficiency is an autosomal recessive inborn error of amino acid metabolism, involving valine, threonine, isoleucine and methionine. This organic aciduria may present in the neonatal period with life-threatening metabolic acidosis, hyperammonemia, feeding difficulties, pancytopenia and coma. Recombinant human MCEE protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

DL-methylmalonyl-CoA racemase, EC 5.1.99.1, GLOD2, glyoxalase domain containing 2, methylmalonyl CoA epimerase, methylmalonyl-CoA epimerase, mitochondrial

Gene Symbol

MCEE

Additional MCEE Products

Product Documents for Recombinant Human MCEE His Protein

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COA

Product Specific Notices for Recombinant Human MCEE His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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