Recombinant Human MDH2 His Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-48325
Key Product Details
Source
E. coli
Tag
His
Conjugate
Unconjugated
Applications
Enzyme Activity, SDS-PAGE
Product Specifications
Description
A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 25-338 of Human MDH2
Source: E.coli
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAKVAVLGAS GGIGQPLSLL LKNSPLVSRL TLYDIAHTPG VAADLSHIET KAAVKGYLGP EQLPDCLKGC DVVVIPAGVP RKPGMTRDDL FNTNATIVAT LTAACAQHCP EAMICVIANP VNSTIPITAE VFKKHGVYNP NKIFGVTTLD IVRANTFVAE LKGLDPARVN VPVIGGHAGK TIIPLISQCT PKVDFPQDQL TALTGRIQEA GTEVVKAKAG AGSATLSMAY AGARFVFSLV DAMNGKEGVV ECSFVKSQET ECTYFSTPLL LGKKGIEKNL GIGKVSSFEE KMISDAIPEL KASIKKGEDF VKTLK
Purity
>95%, by SDS-PAGE
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Predicted Molecular Mass
35.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
Specific activity is > 700 units/mg, and is defined as the amount of enzyme that cleaves 1umole of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH8.0 at 37C.
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Human MDH2 His Protein
![SDS-PAGE: Recombinant Human MDH2 His Protein [NBP1-48325]](https://resources.bio-techne.com/images/products/Recombinant-Human-MDH2-Protein-SDS-Page-NBP1-48325-img0002.jpg "SDS-PAGE: Recombinant Human MDH2 His Protein [NBP1-48325]")
SDS-PAGE: Recombinant Human MDH2 His Protein [NBP1-48325]
SDS-Page: Recombinant Human MDH2 Protein [NBP1-48325] - 15% SDS-PAGE (3 ug).Formulation, Preparation and Storage
NBP1-48325
| Formulation | 20 mM Tris-HCl buffer (pH 7.5), 10% glycerol |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Background: MDH2
Long Name
Malate dehydrogenase, mitochondrial
Alternate Names
EC 1.1.1.37
Gene Symbol
MDH2
Additional MDH2 Products
Product Documents for Recombinant Human MDH2 His Protein
Product Specific Notices for Recombinant Human MDH2 His Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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