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Recombinant Human MIA Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61365

Novus Biologicals, part of Bio-Techne
Catalog #
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NBP2-61365-1000ug
NBP2-61365-100ug
NBP2-61365-500ug
NBP2-61365-5ug

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 107 amino acids corresponding to MIA Source: E. coli

Uniprot ID: Q16674

Amino Acid Sequence: GPMPKLADRK LCADQECSHP ISMAVALQDY MAPDCRFLTI HRGQVVYVFS KLKGRGRLFW GGSVQGDYYG DLAARLGYFP SSIVREDQTL KPGKVDVKTD KWDFYCQ

Purity

>98%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of MIA as determined by LAL method.

Predicted Molecular Mass

12.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human A375 cell line is less than 5 ug/ml, corresponding to a specific activity of > 200 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human MIA Protein

Recombinant Human MIA Protein

SDS-Page: Recombinant Human MIA Protein [NBP2-61365]

SDS-Page: Recombinant Human MIA Protein [NBP2-61365]

Formulation, Preparation and Storage

NBP2-61365
Formulation Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative 5% Trehalose
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: MIA

Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, includinggliomas

Long Name

Melanoma Inhibitory Activity

Alternate Names

CD-RAP

Gene Symbol

MIA

Additional MIA Products

Product Documents for Recombinant Human MIA Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human MIA Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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