Recombinant Human Midkine Protein

Name: Recombinant Human Midkine Protein
Brand: Novus Biologicals
SKU: NBP2-34997

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34997

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Product Specifications
Scientific Data
Preparation & Storage
Background
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Specific Notices

Key Product Details

Source

E. coli

Accession #

P21741

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all Midkine Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 123 amino acids corresponding to Midkine Source: E. coli

Uniprot ID: P21741

Amino Acid Sequence: VAKKKDKVKK GGPGSECAEW AWGPCTPSSK DCGVGFREGT CGAQTQRIRC RVPCNWKKEF GADCKYKFEN WGACDGGTGT KVRQGTLKKA RYNAQCQETI RVTKPCTPKT KAKAKAKKGK GKD

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of Midkine as determined by LAL method.

Predicted Molecular Mass

13.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Midkine Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 0.1-10 ng/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Midkine Protein

SDS-PAGE: Recombinant Human Midkine Protein [NBP2-34997]

SDS-Page: Human Midkine Protein [NBP2-34997]

Formulation, Preparation and Storage

NBP2-34997

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: Midkine

Midkine, also named MK, MK1, NEGF 2, is belonging to the neurotrophic and developmentally-regulated heparin-binding molecules family. It is encoded by the MDK gene. The Midkine protein includes five intrachain disulfide bonds which hold two domains and there are three antiparallel beta-sheets in each domain. A chondroitin sulfate proteoglycan, protein-tyrosine phosphatase zeta (PTPzeta), is a receptor for MK. MK promotes the growth, survival, and migration of various cells, and plays roles in neurogenesis and epithelial mesenchymal interactions during organogenesis. The predicted molecular weight is approximately 13.3 kDa, based on a mature peptide length of 118 amino acid residues in the mouse and 121 amino acid residues in the human. Across species, MK shows 87 % identity between the human and murine proteins.

Alternate Names

ARAP, MDK, MEK, MK1, NEGF2

Gene Symbol

MDK

UniProt