Recombinant Human MMP-14/MT1-MMP Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61379

Novus Biologicals, part of Bio-Techne
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NBP2-61379-100ug
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NBP2-61379-500ug
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Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

SDS-PAGE

View all MMP-14/MT1-MMP Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 264 amino acids corresponding to MMP-14/MT1-MMP Source: E. coli

Uniprot ID: P50281

Amino Acid Sequence: ALASLGSAQS SSFSPEAWLQ QYGYLPPGDL RTHTQRSPQS LSAAIAAMQK FYGLQVTGKA DADTMKAMRR PRCGVPDKFG AEIKANVRRK RYAIQGLKWQ HNEITFCIQN YTPKVGEYAT YEAIRKAFRV WESATPLRFR EVPYAYIREG HEKQADIMIF FAEGFHGDST PFDGEGGFLA HAYFPGPNIG GDTHFDSAEP WTVRNEDLNG NDIFLVAVHE LGHALGLEHS SDPSAIMAPF YQWMDTENFV LPDDDRRGIQ QLYG

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of MMP-14/MT1-MMP as determined by LAL method.

Predicted Molecular Mass

29.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Test in Process.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human MMP-14/MT1-MMP Protein

SDS-PAGE: Recombinant Human MMP-14/MT1-MMP Protein [NBP2-61379]

SDS-PAGE: Recombinant Human MMP-14/MT1-MMP Protein [NBP2-61379]

SDS-Page: Recombinant Human MMP-14/MT1-MMP Protein [NBP2-61379] - SDS-Page of Recombinant Human MMP-14/MT1-MMP Protein under reducing and non-reducing conditions

Formulation, Preparation and Storage

NBP2-61379
Formulation 0.2 um filtered solution in 20 mM Tris-HCl, pH 7.4, 300 mM NaCl, 3 mM CaCl2, 10 uM ZnCl2
Preservative 30% glycerol
Concentration Please see the vial label for concentration. If unlisted please contact technical services.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20C. Avoid freeze-thaw cycles.

Background: MMP-14/MT1-MMP

The matrix metalloproteinase (MMP) family (approximately 25 members in mammals) has been implicated in extracellular matrix remodeling associated with embryonic development, cancer formation and progression, and various other physiological and pathological events. MMP-14 is a membrane-bound matrix metalloproteinase (MT-MMP) capable of mediating pericellular proteolysis of extracellular matrix components. MMP-14 is therefore thought to be an important molecular tool for cellular remodeling of the surrounding matrix (1). Gelatinase A (type-IV collagenase; M(r) 72,000) is produced by tumour stroma cells and is believed to be crucial for their invasion and metastasis, acting by degrading extracellular matrix macro-molecules such as type IV collagen. MMP-14 may thus trigger invasion by tumor cells by activating pro-gelatinase A on the tumor cell surface (2). Observations identify MT1-MMP as a tumor-derived growth factor that regulates proliferation by controlling cell geometry within the confines of the 3D extracellular matrix (ECM) (3).

Long Name

Matrix Metalloproteinase 14/Membrane Type 1 MMP

Alternate Names

MMP-X1, MMP14, MT-MMP1, MT1-MMP

Gene Symbol

MMP14

Additional MMP-14/MT1-MMP Products

Product Documents for Recombinant Human MMP-14/MT1-MMP Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human MMP-14/MT1-MMP Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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