Recombinant Human MRPL13 His Protein

Name: Recombinant Human MRPL13 His Protein
Brand: Novus Biologicals
SKU: NBP2-23240

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23240

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Scientific Data
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all MRPL13 Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-178 of Human MRPL13

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSSFSRA PQQWATFARI WYLLDGKMQP PGKLAAMASI RLQGLHKPVY HALSDCGDHV VIMNTRHIAF SGNKWEQKVY SSHTGYPGGF RQVTAAQLHL RDPVAIVKLA IYGMLPKNLH RRTMMERLHL FPDEYIPEDI LKNLVEELPQ PRKIPKRLDE YTQEEIDAFP RLWTPPEDYR L

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

23.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human MRPL13 His Protein

SDS-PAGE: Recombinant Human MRPL13 His Protein [NBP2-23240]

SDS-Page: MRPL13 Protein [NBP2-23240]

Formulation, Preparation and Storage

NBP2-23240

Formulation	20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MRPL13

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. MRPL13 is a 39S subunit protein. Recombinant human MRPL13 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Alternate Names

39S ribosomal protein L13, mitochondrial, L13, L13A, L13mtRPML13, mitochondrial ribosomal protein L13, MRP-L13, RPL13

Gene Symbol

MRPL13

Additional MRPL13 Products

Product Documents for Recombinant Human MRPL13 His Protein

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COA

Product Specific Notices for Recombinant Human MRPL13 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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