A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 1-95 of Mycobacterium tuberculosis Mycobacterium Tuberculosis ESAT6

Source: Baculovirus

Amino Acid Sequence: ADPMTEQQWN FAGIEAAASA IQGNVTSIHS LLDEGKQSLT KLAAAWGGSG SEAYQGVQQK WDATATELNN ALQNLARTIS EAGQAMASTE GNVTGMFAHH HHHH

Purity

>90%, by SDS-PAGE

Endotoxin Level

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Predicted Molecular Mass

11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mycobacterium Tuberculosis ESAT6 His Protein

SDS-PAGE: Recombinant Mycobacterium Tuberculosis ESAT6 His Protein [NBP1-99045]

SDS-Page: Recombinant Mycobacterium Tuberculosis ESAT6 Protein [NBP1-99045] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Formulation, Preparation and Storage

NBP1-99045

Formulation	20 mM Tris-HCl buffer (pH 8.0), 10% glycerol
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Mycobacterium Tuberculosis ESAT6

ESAT6, the 6 kDa early secretory antigenic target of Mycobacterium tuberculosis, is a secretory protein and potent T cell antigen. ESAT6 has been shown to directly bind to the TLR2 receptor, inhibiting downstream signal transduction. Recently it was shown that ESAT6 is very potential as diagnostic for differentiation between the mycobacterial infection and BCG vaccination. Recombinant Mycobacterium tuberculosis CDC1551 ESAT6 protein, fused to His-tag at C-terminus, was expressed in Baculovirus and purified by using conventional chromatography techniques.

Alternate Names

6 kDa early secretory antigenic target, 6 kDa early secretory antigenic target ESXA, ESAT 6, esaT6, ESXA

Additional Mycobacterium Tuberculosis ESAT6 Products

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COA

Product Specific Notices for Recombinant Mycobacterium Tuberculosis ESAT6 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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Recombinant Mycobacterium Tuberculosis ESAT6 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-99045

Key Product Details

Source

Tag

Conjugate

Applications

View all Mycobacterium Tuberculosis ESAT6 Proteins and Enzymes »

Product Specifications

Description