Recombinant Human N-Acetyl-D-Glucosamine Kinase/NAGK His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-99050

Novus Biologicals, part of Bio-Techne
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NBP1-99050-0.5mg
NBP1-99050-100ug
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all N-Acetyl-D-Glucosamine Kinase/NAGK Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-344 of Human N-Acetyl-D-Glucosamine Kinase/NAGK

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAAIYGG VEGGGTRSEV LLVSEDGKIL AEADGLSTNH WLIGTDKCVE RINEMVNRAK RKAGVDPLVP LRSLGLSLSG GDQEDAGRIL IEELRDRFPY LSESYLITTD AAGSIATATP DGGVVLISGT GSNCRLINPD GSESGCGGWG HMMGDEGSAY WIAHQAVKIV FDSIDNLEAA PHDIGYVKQA MFHYFQVPDR LGILTHLYRD FDKCRFAGFC RKIAEGAQQG DPLSRYIFRK AGEMLGRHIV AVLPEIDPVL FQGKIGLPIL CVGSVWKSWE LLKEGFLLAL TQGREIQAQN FFSSFTLMKL RHSSALGGAS LGARHIGHLL PMDYSANAIA FYSYTFS

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

39.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human N-Acetyl-D-Glucosamine Kinase/NAGK His Protein

SDS-PAGE: Recombinant Human N-Acetyl-D-Glucosamine Kinase/NAGK His Protein [NBP1-99050]

SDS-PAGE: Recombinant Human N-Acetyl-D-Glucosamine Kinase/NAGK His Protein [NBP1-99050]

SDS-Page: Recombinant Human N-Acetyl-D-Glucosamine Kinase/NAGK Protein [NBP1-99050] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation and Storage

NBP1-99050
Formulation 20 mM Tris-HCl buffer (pH 8.0), 2 mM DTT, 20% glycerol, 200 mM NaCl
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: N-Acetyl-D-Glucosamine Kinase/NAGK

NAGK (N-acetyl-D-glucosamine kinase) belongs to the eukaryotic-type N-acetylglucosamine kinase family. NAGK converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. It is a prominent salvage enzyme of amino sugar metabolism in mammals. NAGK has been shown to interact with STK16 and LNX1. Also it has ManNAc kinase activity. Recombinant human NAGK protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

N-Acetyl-D-Glucosamine Kinase

Alternate Names

GlcNAc Kinase, GNK, N-Acetylglucosamine Kinase

Gene Symbol

NAGK

Additional N-Acetyl-D-Glucosamine Kinase/NAGK Products

Product Documents for Recombinant Human N-Acetyl-D-Glucosamine Kinase/NAGK His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human N-Acetyl-D-Glucosamine Kinase/NAGK His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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