Recombinant Human NCK1 His Protein

Name: Recombinant Human NCK1 His Protein
Brand: Novus Biologicals
SKU: NBP1-45253

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-45253

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Product Specifications
Scientific Data
Preparation & Storage
Background
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Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all NCK1 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-377 of Human NCK1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAEEVVVVAK FDYVAQQEQE LDIKKNERLW LLDDSKSWWR VRNSMNKTGF VPSNYVERKN SARKASIVKN LKDTLGIGKV KRKPSVPDSA SPADDSFVDP GERLYDLNMP AYVKFNYMAE REDELSLIKG TKVIVMEKCS DGWWRGSYNG QVGWFPSNYV TEEGDSPLGD HVGSLSEKLA AVVNNLNTGQ VLHVVQALYP FSSSNDEELN FEKGDVMDVIEKPENDPEWW KCRKINGMVG LVPKNYVTVM QNNPLTSGLE PSPPQCDYIR PSLTGKFAGN PWYYGKVTRH QAEMALNERG HEGDFLIRDS ESSPNDFSVS LKAQGKNKHF KVQLKETVYC IGQRKFSTME ELVEHYKKAP IFTSEQGEKL YLVKHLS

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human NCK1 His Protein

SDS-PAGE: Recombinant Human NCK1 His Protein [NBP1-45253]

SDS-Page: NCK1 Protein [NBP1-45253] - 15% SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-45253

Formulation	20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 20% glycerol, 1 mM EDTA, 50 mM NaCl
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NCK1

NCK adaptor protein 1, also known as NCK, belongs to the adaptor family of proteins. The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Overexpression of Nck resulted in transformation of NIH 3T3 and 3Y1 rat fibroblast. Recombinant human NCK1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

Non-catalytic Region of Tyrosine Kinase Adaptor Protein 1

Alternate Names

cytoplasmic protein NCK1, MGC12668, NCK adaptor protein 1melanoma NCK protein, nck-1, NCKalpha, NCKNCK tyrosine kinase, non-catalytic region of tyrosine kinase, SH2/SH3 adaptor protein NCK-alpha

Gene Symbol

NCK1

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COA

Product Specific Notices for Recombinant Human NCK1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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