Recombinant Human NCK2 His Protein

Name: Recombinant Human NCK2 His Protein
Brand: Novus Biologicals
SKU: NBP2-23272

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23272

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all NCK2 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-380 of Human NCK2

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMTEEVIV IAKWDYTAQQ DQELDIKKNE RLWLLDDSKT WWRVRNAANR TGYVPSNYVE RKNSLKKGSL VKNLKDTLGL GKTRRKTSAR DASPTPSTDA EYPANGSGAD RIYDLNIPAF VKFAYVAERE DELSLVKGSR VTVMEKCSDG WWRGSYNGQI GWFPSNYVLE EVDEAAAESP SFLSLRKGAS LSNGQGSRVL HVVQTLYPFS SVTEEELNFE KGETMEVIEK PENDPEWWKC KNARGQVGLV PKNYVVVLSD GPALHPAHAP QISYTGPSSS GRFAGREWYY GNVTRHQAEC ALNERGVEGD FLIRDSESSP SDFSVSLKAS GKNKHFKVQL VDNVYCIGQR RFHTMDELVE HYKKAPIFTS EHGEKLYLVR ALQ

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

45.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human NCK2 His Protein

SDS-PAGE: Recombinant Human NCK2 His Protein [NBP2-23272]

SDS-Page: NCK2 Protein [NBP2-23272]

Formulation, Preparation and Storage

NBP2-23272

Formulation	20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 30% glycerol, 1 mM DTT
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NCK2

NCK2 is a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptiona splice variants, encoding different isoforms, have been characterized. Recombinant human NCK2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

GRB4, NCK adaptor protein 2cytoplasmic protein NCK2, Nck-2, NCKbeta, noncatalytic region of tyrosine kinase, beta, SH2/SH3 adaptor protein NCK-beta

Gene Symbol

NCK2

Additional NCK2 Products

Product Documents for Recombinant Human NCK2 His Protein

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COA

Product Specific Notices for Recombinant Human NCK2 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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