Recombinant Human NDUFA5 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23260

Novus Biologicals, part of Bio-Techne
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NBP2-23260
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all NDUFA5 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-116 of Human NDUFA5

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAGVLKK TTGLVGLAVC NTPHERLRIL YTKILDVLEE IPKNAAYRKY TEQITNEKLA MVKAEPDVKK LEDQLQGGQL EEVILQAEHE LNLARKMREW KLWEPLVEEP PADQWKWPI

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

15.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human NDUFA5 His Protein

SDS-PAGE: Recombinant Human NDUFA5 His Protein [NBP2-23260]

SDS-PAGE: Recombinant Human NDUFA5 His Protein [NBP2-23260]

SDS-Page: NDUFA5 Protein [NBP2-23260]

Formulation, Preparation and Storage

NBP2-23260
Formulation 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 10% glycerol, 1 mM DTT
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NDUFA5

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, also known as NDUFA5, belongs to the complex I NDUFA5 subunit family. The human NDUFA5 gene codes for the B13 subunit of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. The high degree of conservation of NDUFA5 extending to plants and fungi indicates its functional significance in the enzyme complex. The protein localizes to the inner mitochondrial membrane as part of the 7 component-containing, water soluble 'iron-sulfur protein' (IP) fraction of complex I, although its specific role is unknown. It is assumed to undergo post-translational removal of the initiator methionine and N-acetylation of the next amino acid. Recombinant human NDUFA5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques

Alternate Names

B13,5 (13kD, B13), DKFZp781K1356, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa, UQOR13FLJ12147

Gene Symbol

NDUFA5

Additional NDUFA5 Products

Product Documents for Recombinant Human NDUFA5 His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human NDUFA5 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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