Recombinant Human Nesfatin-1/Nucleobindin-2 Protein

Name: Recombinant Human Nesfatin-1/Nucleobindin-2 Protein
Brand: Novus Biologicals
SKU: NBP2-35072

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35072

Submit a review

Citations (4)

Product Datasheet / COA

100% Guarantee

Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P80303

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all Nesfatin-1/Nucleobindin-2 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 82 amino acids corresponding to Nesfatin-1/Nucleobindin-2 Source: E. coli

Uniprot ID: P80303

Amino Acid Sequence: VPIDIDKTKV QNIHPVESAK IEPPDTGLYY DEYLKQVIDV LETDKHFREK LQKADIEEIK SGRLSKELDL VSHHVRTKLD EL

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of Nesfatin-1/Nucleobindin-2 as determined by LAL method.

Predicted Molecular Mass

9.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Nucleobindin 2 Protein is fully biologically active when compared to standard. The biological activity is tested by in vivo assay using healthy wild type male mice (C57BL/6J).

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Nesfatin-1/Nucleobindin-2 Protein

SDS-PAGE: Recombinant Human Nesfatin-1/Nucleobindin-2 Protein [NBP2-35072]

SDS-Page: Human Nesfatin-1/Nucleobindin-2 Protein [NBP2-35072]

Formulation, Preparation and Storage

NBP2-35072

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: Nesfatin-1/Nucleobindin-2

Nesfatin is a metabolic polypeptide and is the N-terminal region of the precursor protein, Nucleobindin2 (encoded by NUCB2 gene). It is a naturally occurring protein and originally identified as a hypothalamic neuropeptide. Additionally, Nucleobindin 2 (Nesfatin) can be found in other areas of brain, and in pancreatic islets beta-cells, gastric endocrine cells and adipocytes. It is responsible for regulating appetite and production of body fat. Excess nesfatin-1 in the brain leads to a loss of appetite, less frequent hunger, a 'sense of fullness', and a drop in body fat and weight. A lack of nesfatin-1 in the brain leads to an increase of appetite, more frequent episodes of hunger, an increase of body fat and weight, and the inability to 'feel full'.

Alternate Names

NEFA, Nesfatin1, NUCB2, Nucleobindin-2, Nesfatin, Nesfatin 1, Nesfatin-1

Gene Symbol

NUCB2

UniProt

P80303 (Human)

Additional Nesfatin-1/Nucleobindin-2 Products

Product Documents for Recombinant Human Nesfatin-1/Nucleobindin-2 Protein

Product Datasheets

Download Product Datasheets

COA

Product Specific Notices for Recombinant Human Nesfatin-1/Nucleobindin-2 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Citations
Reviews