Recombinant Human Neuregulin-1 alpha/NRG1 alpha Protein

Name: Recombinant Human Neuregulin-1 alpha/NRG1 alpha Protein
Brand: Novus Biologicals
SKU: NBP2-35093

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35093

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Product Specifications
Scientific Data
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Background
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Specific Notices

Key Product Details

Source

E. coli

Accession #

Q02297

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all Neuregulin-1 alpha/NRG1 alpha Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 65 amino acids corresponding to Neuregulin-1 alpha/NRG1 alpha Source: E. coli

Uniprot ID: Q02297

Amino Acid Sequence: SHLVKCAEKE KTFCVNGGEC FMVKDLSNPS RYLCKCQPGF TGARCTENVP MKVQNQEKAE ELYQK

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of Neuregulin-1 alpha/NRG1 alpha as determined by LAL method.

Predicted Molecular Mass

7.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Neuregulin-1 alpha/NRG1 alpha protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 40 ng/ml, corresponding to a specific activity of > 2.5 x 10^4 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Neuregulin-1 alpha/NRG1 alpha Protein

SDS-PAGE: Recombinant Human Neuregulin-1 alpha/NRG1 alpha Protein [NBP2-35093]

SDS-Page: Human Neuregulin-1 alpha/NRG1 alpha Protein [NBP2-35093]

Formulation, Preparation and Storage

NBP2-35093

Formulation	Lyophilized from a 0.2 um filtered solution in 20 mM PB, pH 6.0, 150 mM NaCl.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: Neuregulin-1 alpha/NRG1 alpha

Neuregulin 1 (NRG1) is in humans encoded by the NRG1 gene. It is one of four proteins belonging to the neuregulin family that act on the EGFR family of receptor. Neuregulin 1 is produced in numerous isoforms by alternative splicing, which allows it to perform a wide variety of functions. All NRG1 isoforms contain an EGF like domain (alpha or beta splice variant that differ in their C terminal region) that is required for their direct binding to the ErbB3 or ErbB4 receptor tyrosine kinases. NRG1 is necessary for cardiac development, structural maintenance, and functional integrity of the heart. NRG1 and its receptor family ErbB can play a beneficial role in the treatment of chronic heart failure (CHF) by promoting survival of cardiac myocytes, improving sarcomeric structure, balancing Ca2+ homeostasis, and enhancing pumping function.

Alternate Names

Neuregulin1 alpha

Gene Symbol

NRG1

UniProt