Recombinant Human Neuritin Protein

Name: Recombinant Human Neuritin Protein
Brand: Novus Biologicals
SKU: NBP2-35073

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35073

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

Q9NPD7

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all Neuritin Proteins and Enzymes »

Product Specifications

Description

A disulfide-linked homodimeric protein containing two 88 amino acid chains corresponding to Neuritin Source: E. coli

Uniprot ID: Q9NPD7

Amino Acid Sequence: AGKCDAVFKG FSDCLLKLGD SMANYPQGLD DKTNIKTVCT YWEDFHSCTV TALTDCQEGA KDMWDKLRKE SKNLNIQGSL FELCGSGN

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of Neuritin as determined by LAL method.

Predicted Molecular Mass

19.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Neuritin protein is fully biologically active when compared to standard. The ED50 as determined by by a cell proliferation assay using rat C6 cells is less than 25 ng/ml, corresponding to a specific activity of >4.0 x 10^4 IU/mg..

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Neuritin Protein

SDS-PAGE: Recombinant Human Neuritin Protein [NBP2-35073]

SDS-Page: Recombinant Human Neuritin Protein [NBP2-35073]

Formulation, Preparation and Storage

NBP2-35073

Formulation	Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: Neuritin

Neuritin also known as NRN1 and CPG15 is a neurotrophic factor, which is expressed in response to induction of neuronal activity by NGF, BDNF, NT3 and other neural stimulators. It promotes neurite outgrowth and especially branching of neuritic processes in primary hippocampal and cortical cells. Recombinant Human Neuritin is a covalently disulfide-linked homodimer, consisting of two 9.7 kDa polypeptide monomers, each containing 88 amino acid residues. It shares 99 % and 97 % a.a. sequence identity with murine and rat neuritin, respectively.

Alternate Names

NRN1

Gene Symbol