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Recombinant Human NHERF-1 GST (N-Term) Protein

Novus Biologicals, part of Bio-Techne | Catalog # H00009368-P01

Novus Biologicals, part of Bio-Techne
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H00009368-P01-25ug
H00009368-P01-10ug

Key Product Details

Source

Wheat germ

Tag

GST (N-Term)

Conjugate

Unconjugated

Applications

ELISA, Affinity Purification, Microarray, Western Blot

Product Specifications

Description

A recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-358 of Human SLC9A3R1

Source: Wheat Germ (in vitro)

Amino Acid Sequence: MSADAAAGAPLPRLCCLEKGPNGYGFHLHGEKGKLGQYIRLVEPGSPAEKAGLLAGDRLVEVNGENVEKETHQQVVSRIRAALNAVRLLVVDPETDEQLQKLGVQVREELLRAQEAPGQAEPPAAAEVQGAGNENEPREADKSHPEQRELRPRLCTMKKGPSGYGFNLHSDKSKPGQFIRSVDPDSPAEASGLRAQDRIVEVNGVCMEGKQHGDVVSAIRAGGDETKLLVVDRETDEFFKKCRVIPSQEHLNGPLPVPFTNGEIQKENSREALAEAALESPRPALVRSASSDTSEELNSQDSPPKQDSTAPSSTSSSDPILDFNISLAMAKERAHQKRSSKRAPQMDWSKKNELFSNL

Purity

>80% by SDS-PAGE and Coomassie blue staining

Predicted Molecular Mass

65.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human NHERF-1 GST (N-Term) Protein

12.5% SDS-PAGE Stained with Coomassie Blue.

Formulation, Preparation and Storage

H00009368-P01
Preparation Method in vitro wheat germ expression system
Formulation 50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
Preservative No Preservative
Concentration Please see the vial label for concentration. If unlisted please contact technical services.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -80C. Avoid freeze-thaw cycles.

Background: NHERF-1

Members of the ezrin (VIL2; MIM 123900)-radixin (RDX; MIM 179410)-moesin (MSN; MIM 309845) (ERM) protein family are highly concentrated in the apical aspect of polarized epithelial cells. These cells are studded with microvilli containing bundles of actin filaments, which must attach to the membrane to assemble and maintain the microvilli. The ERM proteins, together with merlin, the NF2 (MIM 607379) gene product, are thought to be linkers between integral membrane and cytoskeletal proteins, and they bind directly to actin in vitro. Actin cytoskeleton reorganization requires the activation of a sodium/hydrogen exchanger (SLC9A3; MIM 182307). SLC9A3R1 is an ERM-binding protein.[supplied by OMIM]

Alternate Names

EBP50NHERF-1, ezrin-radixin-moesin binding phosphoprotein-50, Ezrin-radixin-moesin-binding phosphoprotein 50, Na(+)/H(+) exchange regulatory cofactor NHE-RF1, Na+/H+ exchange regulatory co-factor, NHERF1solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 1, NHERFNPHLOP2, Regulatory cofactor of Na(+)/H(+) exchanger, sodium/hydrogen exchanger regulatory factor 1, Sodium-hydrogen exchanger regulatory factor 1, solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatoryfactor 1, solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1, Solute carrier family 9 isoform A3 regulatory factor 1

Gene Symbol

NHERF1

Additional NHERF-1 Products

Product Documents for Recombinant Human NHERF-1 GST (N-Term) Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human NHERF-1 GST (N-Term) Protein

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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