Recombinant Human Noggin Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07104
Key Product Details
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Description
A homodimer containing 205 amino acids corresponding to Noggin Source: Insect cell
Uniprot ID: Q13253
Amino Acid Sequence: QHYLHIRPAP SDNLPLVDLI EHPDPIFDPK EKDLNETLLR SLLGGHYDPG FMATSPPEDR PGGGGGAAGG AEDLAELDQL LRQRPSGAMP SEIKGLEFSE GLAQGKKQRL SKKLRRKLQM WLWSQTFCPV LYAWNDLGSR FWPRYVKVGS CFSKRSCSVP EGMVCKPSKS VHLTVLRWRC QRRGGQRCGW IPIQYPIISE CKCSC
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Level
Less than 0.1 EU/ug of Noggin as determined by LAL method.
Predicted Molecular Mass
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
Biological activity measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.04-0.2 ug/mL in the presence of 50 ng/mL of Recombinant Human BMP-4.
Protein / Peptide Type
Recombinant Protein
Formulation, Preparation and Storage
NBP3-07104
| Formulation | Lyophilized from a 0.2 um filtered concentrated solution in PBS (pH 7.4), 0.02% Tween-20. |
| Preservative | 5% Trehalose |
| Concentration | Lyoph |
| Reconstitution | Briefly centrifuge vial prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20C. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: Noggin
Alternate Names
NOG, SYM1, SYNS1, SYNS1A
Gene Symbol
NOG
Additional Noggin Products
Product Documents for Recombinant Human Noggin Protein
Product Specific Notices for Recombinant Human Noggin Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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