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Recombinant Human Norrin/NDP His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23294

Novus Biologicals, part of Bio-Techne
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NBP2-23294

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 25-133 of Human Norrin/NDP

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSKTDSSFI MDSDPRRCMR HHYVDSISHP LYKCSSKMVL LARCEGHCSQ ASRSEPLVSF STVLKQPFRS SCHCCRPQTS KLKALRLRCS GGMRLTATYR YILSCHCEEC NS

Purity

>80%, by SDS-PAGE

Predicted Molecular Mass

14.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Norrin/NDP His Protein

SDS-PAGE: Recombinant Human Norrin/NDP His Protein [NBP2-23294]

SDS-PAGE: Recombinant Human Norrin/NDP His Protein [NBP2-23294]

SDS-Page: Recombinant Human Norrin/NDP His Protein [NBP2-23294] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation and Storage

NBP2-23294
Formulation 20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Norrin

Norrin precursor, also known as NDP, is a secreted regulatory protein. It activates the canonical Wnt signaling pathway through FZD4 and LRP5 coreceptor. This protein plays a central role in retinal vascularization by acting as a ligand for FZD4 that signals via stabilizing beta-catenin (CTNNB1) and activating LEF/TCF-mediated transcriptional programs. It may be involved in a pathway that regulates neural cell differentiation and proliferation. Recombinant human NDP protein, fused to His-tag at N-terminus, was expressed in E.coli.

Alternate Names

NDP

Gene Symbol

NDP

Additional Norrin Products

Product Documents for Recombinant Human Norrin/NDP His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human Norrin/NDP His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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