A Human Notch-1 antibody blocking peptide

Source: Synthetic (Accession #: P46531)

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Purity

>95%

Application Notes

Control peptide should be used at 1.0 ug per 1.0 ul of antiserum in per assay. Notch-1 (cleaved N-term) peptide has been tested in dot blot.

Protein / Peptide Type

Antibody Blocking Peptide

Formulation, Preparation and Storage

NB300-251PEP

Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative	0.01% Sodium Azide
Concentration	Please see the vial label for concentration. If unlisted please contact technical services.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: Notch-1

Notch (hN1, Motch in mouse, Xotch in Xenopus) is a family of large transmembrane receptors involved in cell to cell interactions and cell fate decisions during invertebrate and vertebrate development. A modification of Notch signaling pathway can lead to changes in cellular proliferation, differentiation, and apoptotic events (1). Notch is activated by binding to membrane-bound ligands of the Delta and Serrate/Jagged family. Activated Notch receptor is proteolytically cleaved and the Notch intracellular domain (NICD) is released. The NICD binds to CSL DNA-binding protein and the Mastermind/Lag-3 co-activator in the nucleus which activates target gene expression (2,3). Notch has been implicated in T-cell acute lymphoblastic leukemia (T-All) and Multiple Sclerosis (MS). Also, repression of Notch signaling is commonly seen in cancer (4). In mammals, four different Notch receptors has been identified (Notch 1, 2, 3, and 4). Active Notch1 has been linked to inactivation of p53-dependent transactivation by inhibiting the phosphorylation of p53 protein (5).

Alternate Names

Notch1, TAN1

Gene Symbol

NOTCH1

UniProt

P46531

Additional Notch-1 Products

Product Documents for Notch-1 Antibody Blocking Peptide

Product Datasheets

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COA

SDS

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Product Specific Notices for Notch-1 Antibody Blocking Peptide

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Citations
Reviews

Notch-1 Antibody Blocking Peptide

Novus Biologicals, part of Bio-Techne | Catalog # NB300-251PEP

Key Product Details

Source

Accession #

Conjugate

Applications

View all Notch-1 Proteins and Enzymes »

Product Specifications

Description