Recombinant Mouse NOV/CCN3 Protein

Name: Recombinant Mouse NOV/CCN3 Protein
Brand: Novus Biologicals
SKU: NBP2-35100

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35100

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Product Specifications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

Q64299

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all NOV/CCN3 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 333 amino acids corresponding to NOV/CCN3 Source: E. coli

Uniprot ID: Q64299

Amino Acid Sequence: QVSASLRCPS RCPPKCPSIS PTCAPGVRSV LDGCSCCPVC ARQRGESCSE MRPCDQSSGL YCDRSADPNN QTGICMVPEG DNCVFDGVIY RNGEKFEPNC QYFCTCRDGQ IGCLPRCQLD VLLPGPDCPA PRKVAVPGEC CEKWTCGSDE QGTQGTLGGL ALPAYRPEAT VGVEVSDSSI NCIEQTTEWS ACSKSCGMGV STRVTNRNRQ CEMVKQTRLC IVRPCEQEPE EVTDKKGKKC LRTKKSLKAI HLQFENCTSL YTYKPRFCGV CSDGRCCTPH NTKTIQVEFQ CLPGEIIKKP VMVIGTCTCY SNCPQNNEAF LQDLELKTSR GEI

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of NOV/CCN3 as determined by LAL method.

Predicted Molecular Mass

36.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

CCN3 Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1.0 ug/ml, corresponding to a specific activity of > 1000 IU/mg.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP2-35100

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris-HCl, pH 8.6, 150 mM NaCl.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: NOV/CCN3

CCN3, also named NOV, IGFBP-9 and NovH, is belonging to the CCN (CTGF/CYR61/NOV) family and it is encoded by the NOV gene. It is expressed in certain tumors, including Wilm's tumor and most nephroblastomas. CCN3 (NOV) interacts with several proteins and is involved in both internal and external cell signaling. CCN3 (NOV) plays an important role in reducing tumorgenicity and proliferation of certain cancer cell lines. It also been reported to exert proangiogenic activities. Recombinant murine NOV is an 36.4 kDa protein containing 333 amino acid residues and it shares 92% and 82% a.a. identity with rat and human NOV.

Long Name

Nephroblastoma Overexpressed Gene

Alternate Names

CCN3, IGFBP-9

Gene Symbol

CCN3

UniProt