Recombinant Human NT-4 Protein

Name: Recombinant Human NT-4 Protein
Brand: Novus Biologicals
SKU: NBP2-35106

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35106

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P34130

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all NT-4 Proteins and Enzymes »

Product Specifications

Description

A noncovalently linked homodimer of two 140 kDa polypeptide monomers (262 total amino acid residues) corresponding to NT-4 Source: E. coli

Uniprot ID: P34130

Amino Acid Sequence: MGVSETAPAS RRGELAVCDA VSGWVTDRRT AVDLRGREVE VLGEVPAAGG SPLRQYFFET RCKADNAEEG GPGAGGGGCR GVDRRHWVSE CKAKQSYVRA LTADAQGRVG WRWIRIDTAC VCTLLSRTGR A

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of NT-4 as determined by LAL method.

Predicted Molecular Mass

28.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

NT-4 protein is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures is less than 50 ng/ml, corresponding to a specific activity of > 2.0 x 104 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human NT-4 Protein

SDS-PAGE: Recombinant Human NT-4 Protein [NBP2-35106]

SDS-Page: Human NT-4 Protein [NBP2-35106]

Formulation, Preparation and Storage

NBP2-35106

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 5.5.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: NT-4

NT-4 also named as NT-5 is a neuronal and epithelial grow factor belongs to the NGF-beta family. The NT-4 precursor is consisted of a 24 a.a. signal peptide, a 56 a.a. propertied and 130 a.a. NT-4. The mature protein has six Cys amino acid residues and has the relative structure with NT-3, BDNF (sharing about 48 % - 52 % sequence identity). Additionally, it shares 91 % and 95 % a.a. sequence identity with mouse and rat NT-4. NT-4 is mainly expressed in prostate and has low level thymus, placenta, and skeletal muscle. It can binding with the LNGFR and trkB receptors and plays a crucial role in the regulation of survival and the maintenance of peripheral sensory sympathetic neurons. Defect of NT-4 may cause primary open angle glaucoma type 1O.

Long Name

Neurotrophin 4

Alternate Names

NT-5, NT4, NTF4, NTF5

Gene Symbol

NTF4

UniProt