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Recombinant Human NTH1 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-72459

Novus Biologicals, part of Bio-Techne
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NBP1-72459-250ug
NBP1-72459-50ug

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-312 of Human NTH1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MCSPQESGMT ALSARMLTRS RSLGPGAGPR GCREEPGPLR RREAAAEARK SHSPVKRPRK AQRLRVAYEG SDSEKGEGAE PLKVPVWEPQ DWQQQLVNIR AMRNKKDAPV DHLGTEHCYD SSAPPKVRRY QVLLSLMLSS QTKDQVTAGA MQRLRARGLT VDSILQTDDA TLGKLIYPVG FWRSKVKYIK QTSAILQQHY GGDIPASVAE LVALPGVGPK MAHLAMAVAW GTVSGIAVDT HVHRIANRLR WTKKATKSPE ETRAALEEWL PRELWHEING LLVGFGQQTC LPVHPRCHAC LNQALCPAAQ GL

Purity

>80%, by SDS-PAGE

Predicted Molecular Mass

36.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human NTH1 His Protein

SDS-PAGE: Recombinant Human NTH1 His Protein [NBP1-72459]

SDS-PAGE: Recombinant Human NTH1 His Protein [NBP1-72459]

SDS-Page: Recombinant Human NTH1 His Protein [NBP1-72459] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation and Storage

NBP1-72459
Formulation 20 mM Tris-HCl buffer (pH8.0), 40% glycerol, 0.15 M NaCl, 1 mM DTT
Preservative No Preservative
Concentration 0.25 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NTH1

NTHL1, also known as nth endonuclease III-like protein 1, is one of the enzyme that has been shown to act on a large number of oxidatively damaged DNA sites. It has an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. It acts on a damaged strand, 5' from the damaged site and incises damaged DNA at cytosines, thymines and guanines. Recombinant human NTHL1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Long Name

Nth Endonuclease III-like 1

Alternate Names

NTHL1, OCTS3

Gene Symbol

NTHL1

Additional NTH1 Products

Product Documents for Recombinant Human NTH1 His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human NTH1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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