Recombinant Mouse Oncostatin M/OSM Protein

Name: Recombinant Mouse Oncostatin M/OSM Protein
Brand: Novus Biologicals
SKU: NBP2-35112

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35112

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Product Specifications
Scientific Data
Preparation & Storage
Background
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Key Product Details

Source

E. coli

Accession #

P53347

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all Oncostatin M/OSM Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 181 amino acids corresponding to Oncostatin M/OSM Source: E. coli

Uniprot ID: P53347

Amino Acid Sequence: NRGCSNSSSQ LLSQLQNQAN LTGNTESLLE PYIRLQNLNT PDLRAACTQH SVAFPSEDTL RQLSKPHFLS TVYTTLDRVL YQLDALRQKF LKTPAFPKLD SARHNILGIR NNVFCMARLL NHSLEIPEPT QTDSGASRST TTPDVFNTKI GSCGFLWGYH RFMGSVGRVF REWDDGSTRS R

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of Oncostatin M/OSM as determined by LAL method.

Predicted Molecular Mass

20.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Oncostatin M/OSM protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine NIH-3T3 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0 x 10^6 IU/mg

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse Oncostatin M/OSM Protein

SDS-PAGE: Recombinant Mouse Oncostatin M/OSM Protein [NBP2-35112]

SDS-Page: Mouse Oncostatin M/OSM Protein [NBP2-35112]

Formulation, Preparation and Storage

NBP2-35112

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 2 x PBS, pH 7.4.
Preservative	5% Trehalose
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: Oncostatin M/OSM

Oncostatin-M (OSM) is a multifunctional cytokine that belongs to the Interleukin-6 subfamily. Among the family members, OSM is most closely related to leukemia inhibitory factor (LIF) and it in fact utilizes the LIF receptor in addition to its specific receptor in the human. A biologically active OSM receptor has been previously described that consists of a heterodimer of leukemia inhibitory factor receptor (LIFR) and gp130. OSM is synthesized by stimulated T-cells and monocytes. Furthermore, the effects of OSM on endothelial cells suggest a pro-inflammatory role for OSM and endothelial cells possess a large number of OSM receptors. Recombinant murine OSM contains 181 amino acids and has a molecular mass of 20.4 kDa. It has approximately 48 % and 72 % amino acid sequence identity with human and rat OSM.

Alternate Names

MGC20461, oncostatin M, oncostatin-M

Gene Symbol

OSM

UniProt