Recombinant Rat Oncostatin M/OSM Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35280
Key Product Details
Source
E. coli
Conjugate
Unconjugated
Applications
Bioactivity, SDS-PAGE
Product Specifications
Description
A single non-glycosylated polypeptide chain containing 214 amino acids corresponding to Oncostatin M/OSM Source: E. coli
Uniprot ID: Q65Z15
Amino Acid Sequence: KRGCSSSSPK LLSQLKSQAN ITGNTASLLE PYILHQNLNT LTLRAACTEH PVAFPSEDML RQLSKPDFLS TVHATLGRVW HQLGAFRQQF PKIQDFPELE RARQNIQGIR NNVYCMARLL HPPLEIPEPT QADSGTSRPT TTAPGIFQIK IDSCRFLWGY HRFMGSVGRV FEEWGDGSRR SRRHSPLWAW LKGDHRIRPS RSSQSAMLRS LVPR
This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Level
Less than 1 EU/ug of Oncostatin M/OSM as determined by LAL method.
Predicted Molecular Mass
24.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
Oncostatin M Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine NIH-3T3 cells is less than 2.0 ng/ml, corresponding to a specific activity of > 5.0 x 10^5 IU/mg.
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Rat Oncostatin M/OSM Protein
![SDS-PAGE: Recombinant Rat Oncostatin M/OSM Protein [NBP2-35280]](https://resources.bio-techne.com/images/products/Recombinant-Rat-Oncostatin-M-OSM-Protein-SDS-Page-NBP2-35280-img0002.jpg "SDS-PAGE: Recombinant Rat Oncostatin M/OSM Protein [NBP2-35280]")
SDS-PAGE: Recombinant Rat Oncostatin M/OSM Protein [NBP2-35280]
SDS-Page: Recombinant Rat Oncostatin M/OSM Protein [NBP2-35280]Formulation, Preparation and Storage
NBP2-35280
| Formulation | Lyophilized from a 0.2 um filtered concentrated solution in 2 x PBS, 0.1 % Tween-80. |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at |
| Format | Carrier-Free |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: Oncostatin M/OSM
Alternate Names
MGC20461, oncostatin M, oncostatin-M
Gene Symbol
OSM
Additional Oncostatin M/OSM Products
Product Documents for Recombinant Rat Oncostatin M/OSM Protein
Product Specific Notices for Recombinant Rat Oncostatin M/OSM Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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