Recombinant Mouse OX40 Ligand/TNFSF4 Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-26577

Novus Biologicals, part of Bio-Techne
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Key Product Details

Conjugate

Unconjugated

Applications

Functional Assay, SDS-PAGE

View all OX40 Ligand/TNFSF4 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein corresponding to TNFSF4.

Amino Acid Sequence:
EPRGPTIKPCPPCKCPAPNLLGGPSVFIFPPKIKDVLMISLSPIVTCVVVDVSEDDPDVQISWFVNNVEVHTAQTQTHREDYNSTLRVVSALPIQHQDWMSGKEFKCKVNNKDLPAPIERTISKPKGSVRAPQVYVLPPPEEEMTKKQVTLTCMVTDFMPEDIYVEWTNNGKTELNYKNTEPVLDSDGSYFMYSKLRVEKKNWVERNSYSCSVVHEGLHNHHTTKSFSRTPGKSGRQLSSSPAKDPPIQRLRGAVTRCEDGQLFISSYKNEYQTMEVQNNSVVIKCDGLYIIYLKGSFFQEVKIDLHFREDHNPISIPMLNDGRRIVFTVVASLAFKDKVYLTVNAPDTLCEHLQINDGELIVVQLTPGYCAPEGSYHSTVNQVPL Mouse Ig-Fc amino acid sequence, followed by linker (SGR) in bold and Extracellular mouse OX40L amino acid sequence is underlined

Purity

Protein A purified

Endotoxin Level

Endotoxin level < 0.1 EU/ug of the protein (

Predicted Molecular Mass

43.54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

In vitro proliferation assay, in vivo assays.This protein was measured by its ability to co-stimulate IL-2 secretion in the presence of anti-mCD3, and agonist antibody OX86.

Applications

Functional (reported in scientific literature (PMID 24633226))
In vitro assay (reported in scientific literature (PMID 24633226))

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse OX40 Ligand/TNFSF4 Protein

In vitro assay: Recombinant Mouse OX40 Ligand/TNFSF4 Protein [NBP2-26577]

In vitro assay: Recombinant Mouse OX40 Ligand/TNFSF4 Protein [NBP2-26577]

In vitro assay: TNFSF4 Protein [NBP2-26577] - Demonstration in vitro of the potency of Fc-OX40L in comparison to an agonist antibody OX86, with anti-CD3 alone serving as a negative control for co-stimulation.
SDS-PAGE: Recombinant Mouse OX40 Ligand/TNFSF4 Protein [NBP2-26577]

SDS-PAGE: Recombinant Mouse OX40 Ligand/TNFSF4 Protein [NBP2-26577]

SDS-Page: TNFSF4 Protein [NBP2-26577] - Electrophoretic analysis of Fc-mOX40L using Coomassie blue stained 4-20% reducing SDS-PAGE.
SDS-PAGE: Recombinant Mouse OX40 Ligand/TNFSF4 Protein [NBP2-26577]

SDS-PAGE: Recombinant Mouse OX40 Ligand/TNFSF4 Protein [NBP2-26577]

SDS-Page: Recombinant Mouse OX40 Ligand/TNFSF4 Protein [NBP2-26577] - Electrophoretic analysis of Fc-mOX40L using Coomassie blue stained 4-15% reducing SDS-PAGE.

Formulation, Preparation and Storage

NBP2-26577
Formulation Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 um filter sterilized.
Preservative No Preservative
Concentration 2 mg/ml
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -80C. Avoid freeze-thaw cycles.

Background: OX40 Ligand/TNFSF4

OX40 (CD134) and its natural ligand OX40L (CD134L) belong to the tumor necrosis factor superfamily. OX40L is primarily expressed on antigen presenting cells including activated B-cells, dendritic cells, and macrophages. OX40L has been shown to be expressed on activated endothelial cells and CD4+ T-cells during antigen stimulation. OX-40 and OX-40L interactions are crucial for the generation and survival of memory CD4+ T-cells. These interactions may stimulate effector and memory CD4+ and CD8+ T-cells and suppress CD4+/CD25+ Tregs and may have a role in enhanced immune response.

Alternate Names

CD252, gp34, TNFSF4

Gene Symbol

TNFSF4

Additional OX40 Ligand/TNFSF4 Products

Product Documents for Recombinant Mouse OX40 Ligand/TNFSF4 Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse OX40 Ligand/TNFSF4 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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