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Recombinant Human PCNA associated factor His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23168

Novus Biologicals, part of Bio-Techne
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NBP2-23168

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-111 of Human PCNA associated factor

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMVRTKAD SVPGTYRKVV AARAPRKVLG SSTSATNSTS VSSRKAENKY AGGNPVCVRP TPKWQKGIGE FFRLSPKDSE KENQIPEEAG SSGLGKAKRK ACPLQPDHTN DEKE

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

14.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human PCNA associated factor His Protein

SDS-PAGE: Recombinant Human PCNA associated factor His Protein [NBP2-23168]

SDS-PAGE: Recombinant Human PCNA associated factor His Protein [NBP2-23168]

SDS-Page: PCNA associated factor Protein [NBP2-23168]

Formulation, Preparation and Storage

NBP2-23168
Formulation 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 10% glycerol, 1 mM DTT
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PCNA associated factor

KIAA0101 is a PCNA-binding protein that acts as a regulator of DNA repair during DNA replication. Following DNA damage, the interaction with PCNA is disrupted, facilitating the interaction between monoubiquitinated PCNA and the translesion DNA synthesis DNA polymerase eta (POLH) at stalled replisomes, facilitating the bypass of replication-fork-blocking lesions. This protein also acts as a regulator of centrosome number. Recombinant human KIAA0101 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

HCV NS5A-transactivated protein 9, Hepatitis C virus NS5A-transactivated protein 9, KIAA0101, NS5ATP9p15PAF, OEATC-1OEATC1, Overexpressed in anaplastic thyroid carcinoma 1, p15(PAF), PAF, PCNA-associated factor

Gene Symbol

PCLAF

Additional PCNA associated factor Products

Product Documents for Recombinant Human PCNA associated factor His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human PCNA associated factor His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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