Recombinant Human PCNA Protein

Name: Recombinant Human PCNA Protein
Brand: Novus Biologicals
SKU: NBC1-18428

Novus Biologicals, part of Bio-Techne | Catalog # NBC1-18428

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Technical Data
Citations & Reviews

Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

SDS-PAGE

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Product Specifications

Description

An un-tagged recombinant protein corresponding to the amino acids 1-261 of Human PCNA

Source: E.coli

Amino Acid Sequence: MFEARLVQGS ILKKVLEALK DLINEACWDI SSSGVNLQSM DSSHVSLVQL TLRSEGFDTY RCDRNLAMGV NLTSMSKILK CAGNEDIITL RAEDNADTLA LVFEAPNQEK VSDYEMKLMD LDVEQLGIPE QEYSCVVKMP SGEFARICRD LSHIGDAVVI SCAKDGVKFS ASGELGNGNI KLSQTSNVDK EEEAVTIEMN EPVQLTFALR YLNFFTKATP LSSTVTLSMS ADVPLVVEYK IADMGHLKYY LAPKIEDEEG S

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

28.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human PCNA Protein

SDS-PAGE: Recombinant Human PCNA Protein [NBC1-18428]

SDS-Page: Recombinant Human PCNA Protein [NBC1-18428] - PCNA, 28 kDa (261 aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Formulation, Preparation and Storage

NBC1-18428

Formulation	20 mM Tris-HCl buffer (pH 7.5), 2 mM EDTA, 20% glycerol
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PCNA

PCNA (Proliferating Cell Nuclear Antigen) is found in the nucleus and is a cofactor of DNA polymerase delta. This protein is associated with DNA synthesis and repair. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. It appears during late G1- phase, S-phase of mitosis and persists until the end of the M-phase because of its long biological half-life. PCNA may be induced by UV irradiation, growth factors and eventually by neighbouring tumours. Recombinant human PCNA, was expressed in E.coli and purified by conventional chromatography techniques.

Long Name

Proliferating Cell Nuclear Antigen

Alternate Names

Cyclin, DNA polymerase delta auxiliary protein, MGC8367, proliferating cell nuclear antigen

Gene Symbol

PCNA

Additional PCNA Products

Product Documents for Recombinant Human PCNA Protein

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COA

Product Specific Notices for Recombinant Human PCNA Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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