Recombinant Rat PDGF-AA Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35281
Key Product Details
Source
E. coli
Conjugate
Unconjugated
Applications
Bioactivity, SDS-PAGE
Product Specifications
Description
A disulfide-linked homodimeric protein containing two 111 amino acid polypeptide (A chain) chains corresponding to PDGF-AA Source: E. coli
Uniprot ID: P28576
Amino Acid Sequence: MSIEEAIPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATSNLN PDHREEETDV R
Purity
> 98 % pure by SDS-PAGE and HPLC
Endotoxin Level
Less than 0.1 EU/ug of PDGF-AA as determined by LAL method.
Predicted Molecular Mass
25.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
PDGF-AA Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 5.0 ng/ml, corresponding to a specific activity of > 2.0 x 10^5 IU/mg.
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Rat PDGF-AA Protein
![SDS-PAGE: Recombinant Rat PDGF-AA Protein [NBP2-35281]](https://resources.bio-techne.com/images/products/Rat-PDGF-AA-Protein-SDS-Page-NBP2-35281-img0001.jpg "SDS-PAGE: Recombinant Rat PDGF-AA Protein [NBP2-35281]")
SDS-PAGE: Recombinant Rat PDGF-AA Protein [NBP2-35281]
SDS-Page: Rat PDGF-AA Protein [NBP2-35281]Formulation, Preparation and Storage
NBP2-35281
| Formulation | Lyophilized from a 0.2 um filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA. |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in 4mM HCl to a concentration of 0.1-1.0mg/mL. |
| Format | Carrier-Free |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: PDGF-AA
Long Name
Platelet-derived Growth Factor AA
Alternate Names
PDGFAA
Gene Symbol
PDGFA
Additional PDGF-AA Products
Product Documents for Recombinant Rat PDGF-AA Protein
Product Specific Notices for Recombinant Rat PDGF-AA Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...