Recombinant Human PDGF-BB Protein

Name: Recombinant Human PDGF-BB Protein
Brand: Novus Biologicals
SKU: NBP2-35203

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35203

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P01127

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all PDGF-BB Proteins and Enzymes »

Product Specifications

Description

A disulfide-linked homodimeric protein containing two 110 amino acid polypeptide (B chain) chains corresponding to PDGF-BB Source: E. coli

Uniprot ID: P01127

Amino Acid Sequence: MSLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETVAAARPVT

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of PDGF-BB as determined by LAL method.

Predicted Molecular Mass

24.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

PDGFB Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 3 ng/ml, corresponding to a specific activity of > 3.3 x 10^5 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human PDGF-BB Protein

SDS-PAGE: Recombinant Human PDGF-BB Protein [NBP2-35203]

SDS-Page: Human PDGF-B Protein [NBP2-35203]

Formulation, Preparation and Storage

NBP2-35203

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: PDGF-BB

Platelet-derived growth factor (PDGF) presenting in serum but absent from plasma was first discovered in animal study by Lynch and co-workers in the late 1980s. It is a disulfide-linked dimer consisting of two peptides-chain A and chain B. PDGF has three subforms: PDGF-AA, PDGF-BB, PDGF-AB. It is involved in a number of biological processes, including hyperplasia, embryonic neuron development, chemotaxis, and respiratory tubule epithelial cell development. The function of PDGF is mediated by two receptors (PDGFR-alpha and PDGFR-beta).

Long Name

Platelet-derived Growth Factor BB

Alternate Names

PDGFBB

Gene Symbol

PDGFB

UniProt