Recombinant Mouse PDGFA Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43148

Novus Biologicals, part of Bio-Techne
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NBP1-43148-0.025mg
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Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity, ELISA

View all PDGF-A Proteins and Enzymes »

Product Specifications

Description

Recombinant non-glycosylated homodimer of 126 amino acids corresponding to PDGF.

Source: E. coli

Purity

>95% pure by SDS-PAGE

Endotoxin Level

Less than 0.01 ng/ug cytokine as determined by the LAL assay.

Predicted Molecular Mass

28.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

This reagent has been tested in bioassays using the mouse cell line 3T3. The ED50 measured in a 3T3 proliferation assay is typically 10 ng/ml, corresponding to a specific activity of approximately 1 x10^5Units/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse PDGFA Protein

ELISA: Recombinant Mouse PDGFA Protein [NBP1-43148]

ELISA: Recombinant Mouse PDGFA Protein [NBP1-43148]

ELISA: PDGFA Protein [NBP1-43148] - Proliferation of 3T3 cells in response to Mouse PDGF-AA Recombinant Protein

Formulation, Preparation and Storage

NBP1-43148
Formulation PBS (pH 7.2)
Preservative No Preservative
Concentration 0.1 mg/ml
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -80C. Avoid freeze-thaw cycles.

Background: PDGF-A

The platelet-derived growth factor AA (PDGF-AA) is a member of the PDGF family. The PDGF proteins are derived from four genes (PDGF-A, -B, -C, and -D) that form four disulfide-linked homodimers (PDGF-AA, -BB, -CC, and -DD) and one heterodimer (PDGF-AB). PDGF-AA plays an important role as a mitogen for a number of cell types. Recombinant Mouse PDGF-AA produced in e.coli is a non-glycosylated homodimer of 126 amino acids with a molecular weight of 28.9 kDa.

Long Name

Platelet-derived Growth Factor A

Alternate Names

PDGF-1, PDGF1PDGF A-chain, PDGF-A, Platelet-derived growth factor A chain, platelet-derived growth factor alpha chain, platelet-derived growth factor alpha isoform 2 preproprotein, platelet-derived growth factor alpha polypeptidePDGF subunit A, platelet-derived growth factor subunit A

Gene Symbol

PDGFA

Additional PDGF-A Products

Product Documents for Recombinant Mouse PDGFA Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Mouse PDGFA Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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