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Recombinant Human Peroxiredoxin 6 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-30188

Novus Biologicals, part of Bio-Techne
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NBP1-30188

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

Functional Assay, SDS-PAGE

Product Specifications

Description

A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-224 of Human Peroxiredoxin 6

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MPGGLLLGDV APNFEANTTV GRIRFHDFLG DSWGILFSHP RDFTPVCTTE LGRAAKLAPE FAKRNVKLIA LSIDSVEDHL AWSKDINAYN CEEPTEKLPF PIIDDRNREL AILLGMLDPA EKDEKGMPVT ARVVFVFGPD KKLKLSILYP ATTGRNFDEI LRVVISLQLT AEKRVATPVD WKDGDSVMVL PTIPEEEAKK LFPKGVFTKE LPSGKKYLRY TPQP

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

27.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is >2,000 pmol/min/ug. Enzymatic activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25C for 1minute.

Application Notes

Use In vitro reported in scientific literature (PMID 26553463).

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Peroxiredoxin 6 His Protein

SDS-PAGE: Recombinant Human Peroxiredoxin 6 His Protein [NBP1-30188]

SDS-PAGE: Recombinant Human Peroxiredoxin 6 His Protein [NBP1-30188]

SDS-Page: Recombinant Human Peroxiredoxin 6 Protein [NBP1-30188] - Peroxiredoxin 6, 27.1 kDa (244aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Formulation, Preparation and Storage

NBP1-30188
Formulation 20 mM Tris-HCl buffer (pH8.0), 20% Glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Peroxiredoxin 6

Peroxiredoxin 6, also known as PRDX6, is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell and can reduce H2O2 and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. Recombinant human peroxiredoxin 6 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Alternate Names

aiPLA2, AOP2, NSGPx, PRDX6

Gene Symbol

PRDX6

Additional Peroxiredoxin 6 Products

Product Documents for Recombinant Human Peroxiredoxin 6 His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human Peroxiredoxin 6 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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