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Recombinant Human PHPT1 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59581

Novus Biologicals, part of Bio-Techne
Catalog #
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NBP2-59581-100ug
NBP2-59581-20ug

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

Enzyme Activity, SDS-PAGE

Product Specifications

Description

A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-125 of Human PHPT1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAVADLALIP DVDIDSDGVF KYVLIRVHSA PRSGAPAAES KEIVRGYKWA EYHADIYDKV SGDMQKQGCD CECLGGGRIS HQSQDKKIHV YGYSMAYGPA QHAISTEKIK AKYPDYEVTW ANDGY

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

15.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is >120 units/mg, and is defined as the amount of enzyme that hydrolyze 1nmol f p-nitrophenyl phosphate per minute at pH 7.5 at 37C.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human PHPT1 His Protein

SDS-PAGE: Recombinant Human PHPT1 His Protein [NBP2-59581]

SDS-PAGE: Recombinant Human PHPT1 His Protein [NBP2-59581]

SDS-Page: Recombinant Human PHPT1 Protein [NBP2-59581] - 15% SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP2-59581
Formulation 20 mM Tris-HCl buffer (pH8.0), 0.2 M NaCl, 2 mM DTT, 10% glycerol
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PHPT1

PHPT1 is an EDTA-insensitive phosphohistidine phosphatase that catalyzes the dephosphorylation of phosphopeptide I (Ek et al., 2002 (PubMed 12383260)).(supplied by OMIM)

Alternate Names

bA216L13.10, DKFZp564M173, EC 3.1.3,14 kDa phosphohistidine phosphatase, EC 3.1.3.-, HSPC141, phosphohistidine phosphatase 11700008C22Rik, phosphohistidine phosphatase 14kDa, PHP14CGI-202, Protein janus-A homolog, RP11-216L13.10, sex-regulated protein janus-a

Gene Symbol

PHPT1

Additional PHPT1 Products

Product Documents for Recombinant Human PHPT1 His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human PHPT1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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