We have a customer who purchased NBP1-78446 and NBP1-78446PEP (PO12-01725/PO12-01822). The customer uses the antibody at a dilution of 1:10,000 in IHC successfully, but would like to use the blocking peptide to verify their IHC results. The customer would like to receive the recommended blocking peptide protocol for NBP1-78446PEP - most important, which is the recommended concentration/fold relation to antibody the customer should use!

We have a general recommended protocol for blocking peptides, which should be useful for you. You will see that in point 2., we say 'add the blocking peptide solution at a 5-10:1 ratio to your antibody, i.e. if you added 1 ug of antibody, add 5-10 ug of blocking peptide'.

I received the Piezo-1 blocking peptide this morning. In the past, I have blocked antibodies for IHC by pre-absorbing the antibody with a 5-fold excess of blocking peptide for 2 hours at 4 degrees C before diluting the blocked mixture and applying to the slides. Can you confirm that this protocol is what you would recommend for effective blocking, or otherwise, suggest appropriate conditions. I am also assuming from the data sheet you sent that you recommend to reconstitute the peptide in water.

The peptide you received is not lyophilized but in solution form and the protocol you mentioned seems great.

PIEZO1 Antibody Blocking Peptide

Name: PIEZO1 Antibody Blocking Peptide
Brand: Novus Biologicals
SKU: NBP1-78446PEP

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-78446PEP

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Product Datasheet / COA

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Product Specifications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Conjugate

Unconjugated

Applications

Antibody Competition

View all PIEZO1 Proteins and Enzymes »

Product Specifications

Description

A PIEZO1 antibody blocking peptide.

Application Notes

This peptide is useful as a blocking peptide for NBP1-78446. For further blocking peptide related protocol, click here.

Specificity

This peptide is specific for NBP1-78446 only.

Protein / Peptide Type

Antibody Blocking Peptide

Formulation, Preparation and Storage

NBP1-78446PEP

Formulation	Peptide dissolved in dH2O. Contains no BSA.
Preservative	No Preservative
Concentration	1.0 mg/ml
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -80C. Avoid freeze-thaw cycles.

Background: PIEZO1

PIEZO1 (piezo-type mechanosensitive ion channel component 1) plays a crucial role in epithelial cell adhesion. PIEZO1 belongs to the PIEZO family of transmembrane proteins. Mutations in Piezo1 have been tied to Xerocytosis. PIEZO1 has been studied in relation to neuronitis, Alzheimer's disease and Parkinson's disease.

Long Name

Piezo-type mechanosensitive ion channel component 1

Alternate Names

FAM38A, KIAA0233, Mib, Protein FAM38A

Gene Symbol

PIEZO1

Additional PIEZO1 Products

Product Documents for PIEZO1 Antibody Blocking Peptide

Product Datasheets

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COA

Product Specific Notices for PIEZO1 Antibody Blocking Peptide

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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