Recombinant Human PKI-alpha GST (N-Term) Protein

Name: Recombinant Human PKI-alpha GST (N-Term) Protein
Brand: Novus Biologicals
SKU: H00005569-P01

Novus Biologicals, part of Bio-Techne | Catalog # H00005569-P01

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Product Datasheet / COA / SDS

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

Wheat germ

Tag

GST (N-Term)

Conjugate

Unconjugated

Applications

ELISA, Affinity Purification, Microarray, SDS-PAGE, Western Blot

View all PKI-alpha Proteins and Enzymes »

Product Specifications

Description

Recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-76 of Human PKIA

Source: Wheat Germ (in vitro)

Amino Acid Sequence: MTDVETTYADFIASGRTGRRNAIHDILVSSASGNSNELALKLAGLDINKTEGEEDAQRSSTEQSGEAQGEAAKSES

Purity

>80% by SDS-PAGE and Coomassie blue staining

Predicted Molecular Mass

34.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human PKI-alpha GST (N-Term) Protein

SDS-PAGE: Recombinant Human PKI-alpha GST (N-Term) Protein [H00005569-P01]

SDS-Page: Recombinant Human PKI-alpha Protein [H00005569-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.

Formulation, Preparation and Storage

H00005569-P01

Preparation Method	in vitro wheat germ expression system
Formulation	50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
Preservative	No Preservative
Concentration	Please see the vial label for concentration. If unlisted please contact technical services.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -80C. Avoid freeze-thaw cycles.

Background: PKI-alpha

The protein encoded by this gene is a member of the cAMP-dependent protein kinase (PKA) inhibitor family. This protein was demonstrated to interact with and inhibit the activities of both C alpha and C beta catalytic subunits of the PKA. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq]

Alternate Names

cAMP-dependent protein kinase inhibitor alpha, cAMP-dependent protein kinase inhibitor, muscle/brain isoform, PKI-alpha, PRKACN1, protein kinase (cAMP-dependent, catalytic) inhibitor alpha

Gene Symbol

PKIA

Additional PKI-alpha Products

Product Documents for Recombinant Human PKI-alpha GST (N-Term) Protein

Product Datasheets

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COA

SDS

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Product Specific Notices for Recombinant Human PKI-alpha GST (N-Term) Protein

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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