ProDots Recombinant Human IL-7 GMP Protein GMP

Name: ProDots Recombinant Human IL-7 GMP Protein
Brand: R&D Systems
SKU: PRD207-GMP

R&D Systems, part of Bio-Techne | Catalog # PRD207-GMP

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Product Datasheet / COA / SDS

10 µg of GMP rhIL-7 supplied as 2 protein balls (each 5 µg) in a bag

Discontinued Product

PRD207-GMP has been discontinued. View all IL-7 products.

Key Product Details

Source:	E. coli
Accession #:	P13232.1
Applications:	Bioactivity

View Full Technical Details for ProDots Recombinant Human IL-7 GMP Protein

Technical Data
Citations / Reviews

Product Specifications for ProDots Recombinant Human IL-7 GMP Protein

Source

E. coli-derived
Asp26-His177, with an N-terminal Met
Produced using non-animal reagents in an animal-free laboratory.
Manufactured and tested under cGMP guidelines.

Purity

>97%, by SDS-PAGE with silver staining, under reducing conditions.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Met-Asp-(Cys)-Asp-Ile-Glu-Gly-Lys-Asp-Gly

Predicted Molecular Mass

17 kDa

SDS-PAGE

17 kDa

Activity

Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL). Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. USA 83:5894. The ED₅₀ for this effect is 0.1-0.5 ng/mL.

The specific activity of ProDots Recombinant Human IL-7 GMP is >1.0 x 10⁸ units/mg, which is calibrated against the human IL-7 reference standard (NIBSC code: 90/530).

Scientific Data Examples for ProDots Recombinant Human IL-7 GMP Protein

	10 µg of GMP Human IL-7 as two spheroids each 5 µg inside a sterile bag with a needless valve port and weldable tube port. GMP-grade Prodots® Recombinant Human IL-7 stimulates proliferation of PHA-activated human peripheral blood lymphocytes. The ED₅₀ for this effect is 0.1-0.5 ng/mL.
	Equivalent bioactivity of GMP ProDot™ Proteins to GMP Proteins Comparative tests for function within biologically relevant model systems show equivalence of GMP ProDot™ Proteins and GMP Proteins. Activation of PBMCs after 9 days in culture with ExCellerate™ Human T Cell Media (CCM030), GMP Cloudz Human T Cell Activation Kit (CLD001-GMP) and GMP IL-7 ProDot (PRD247-GMP) or GMP IL-7 protein (247-GMP) shows comparable A. T cell fold expansion and B. phenotypes.

Formulation, Preparation and Storage

PRD207-GMP

Formulation	Lyophilized from an phosphate buffered saline-based formulation using proprietary excipients.
Reconstitution	Reconstitute immediately prior to use with up to 25 mL of cell culture media.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Store material as supplied, unopened, and unreconstituted at 2-8 °C until use. Stability is a minimum of 8 weeks when stored at 2-8 °C as supplied. Refer to lot specific COA for the Use by Date. The foil pouch is used for shipping and storage only and is not terminally sterilized. GMP ProDots within the weldable bag are prepared under GMP-controlled conditions and protein content is tested to USP <71> guidelines.

Background: IL-7

IL-7 (interleukin-7) is a 25 kDa cytokine of the hemopoietin family that plays important roles in lymphocyte differentiation, proliferation, and survival (1-4). Human IL‑7 cDNA encodes 177 amino acids (aa) that include a 25 aa signal peptide (3). Human IL-7 shares approximately 60-63% aa sequence identity with mouse, rat, canine and feline IL-7, and 72-76% with equine, bovine, ovine, and porcine IL-7. Human and mouse IL-7 exhibit cross-species activity (2, 3). IL-7 is produced by a wide variety of cells in primary and secondary lymphoid tissues, including stromal epithelial cells of the thymus, bone marrow, and intestines (1, 2, 5). Circulating IL-7 is limiting in healthy animals, but increases during lymphopenia (1, 6). IL-7 signals through a complex of the IL-7 Receptor alpha subunit (IL-7 R alpha, also known as CD127) with the common gamma chain ( gamma c) (1). The gamma c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21 (1). IL-7 R alpha is expressed on double negative (CD4-CD8-) and single positive (CD4+ or CD8+) naïve and memory T cells, but undergoes IL-7-mediated down‑regulation and shedding during antigen-driven T cell proliferation, and is absent on regulatory T cells (1, 2, 6-11). IL-7 contributes to the maintenance of all naïve and memory T cells, mainly by promoting expression of the anti-apoptotic protein Bcl-2 (9-11). It is required for optimal T cell-dendritic cell interaction (6). IL-7 is expressed early in B cell development prior to the appearance of surface IgM (1, 5, 9). In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development, while in humans, it is required for T cell but not for B cell development (4, 9, 12, 13). However, IL-7 functions in both mouse and human pro-B cells to suppress premature Ig light chain recombination during proliferative growth (14, 15).

Item	Value
References	Sasson, S.C. et al. (2006) Curr. Drug Targets 7:1571. Barata, J.T. et al. (2006) Exp. Hematol. 34:1133. Goodwin, R.G. et al. (1990) Proc. Natl. Acad. Sci. USA 86:302. Namen, A.E. et al. (1988) Nature 333:571. Shalapour, S. et al. (2012) PLoS ONE 7: e31939. Saini, M. et al. (2009) Blood 113:5793. Park, J.H. et al. (2004) Immunity 21:289. Vranjkovic, A. et al. (2007) Int. Immunol. 19:1329. Sudo, T. et al. (1993) Proc. Natl. Acad. Sci. 90:9125. Seddon, B. et al. (2003) Nat. Immunol. 4:680. Schluns, K.S. et al. (2000) Nat. Immunol. 5:426. Peschon, J.J. et al. (1994) J. Exp. Med. 180:1955. Pribyl, J.A. and T.W. LeBien (1996) Proc. Natl. Acad. Sci. 93:10348. Johnson, K. et al. (2012) J. Immunol. 188:6084. Nodland, S.E. et al. (2011) Blood 118:2116.
Long Name	Interleukin 7
Entrez Gene IDs	3574 (Human); 16196 (Mouse); 25647 (Rat)
Alternate Names	IL-7, IL-7interleukin-7, IL7, Lymphopoietin-1, PBGF, interleukin 7

Product Specific Notices for ProDots Recombinant Human IL-7 GMP Protein

The GMP ProDot bag(s) should be used after proper inspection. Assess the bags after media is added to the bag either through the weldable port or needless valve. Inspect the bag to ensure complete dissolution of the GMP ProDot, and visually inspect seals and corners for a complete seal.

GMP ProDots are fragile. Please handle with care. If breakage of a ProDot is observed, there is no integrity lost, and can be used as indicated.

SDS-PAGE Purity, Host Cell DNA, and Host Cell Protein testing were completed on protein prior to lyophilization.

The End User is aware that R&D Systems, Inc. sells GMP products for preclinical or clinical ex vivo use and not for in vivo use. The End User Terms of Use of Product may be found at: RnDSystems.com/Legal .

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ProDots Recombinant Human IL-7 GMP Protein GMP

R&D Systems, part of Bio-Techne | Catalog # PRD207-GMP

Key Product Details

Product Specifications for ProDots Recombinant Human IL-7 GMP Protein

Scientific Data Examples for ProDots Recombinant Human IL-7 GMP Protein

Formulation, Preparation and Storage

Background: IL-7

Product Specific Notices for ProDots Recombinant Human IL-7 GMP Protein

Customer Reviews for ProDots Recombinant Human IL-7 GMP Protein

Product Documents for ProDots Recombinant Human IL-7 GMP Protein

Product Datasheets

COA

Certificate of Analysis

SDS

CONTACT INFORMATION

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